Performance of the multitarget Mikrogen Chlamydia trachomatis IgG ELISA in the prediction of tubal factor infertility (TFI) in subfertile women: comparison with the Medac MOMP IgG ELISA plus

被引：5
作者：
van Ess, Eleanne F. [1 ]
Ouburg, Sander [1 ]
Spaargaren, Joke [1 ]
Land, Jolande A. [2 ]
Morre, Servaas A. [1 ,3 ]
机构：
[1] Vrije Univ Amsterdam, Med Ctr, Dept Med Microbiol & Infect Control, Lab Immunogenet, Room O-2-6E59,De Boelelaan 1108,POB 7057, NL-1007 MB Amsterdam, Netherlands
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Obstet & Gynaecol, NL-9700 RB Groningen, Netherlands
[3] Univ Maastricht, Res Inst GROW, Fac Hlth Med & Life Sci, Inst Publ Hlth Genom IPHG,Dept Genet & Cell Biol, NL-6200 MB Maastricht, Netherlands
来源：
PATHOGENS AND DISEASE | 2017年 / 75卷 / 07期
关键词：
Chlamydia trachomatis; tubal factor infertility; tubal pathology; serology; ELISA; IgG; PATIENT DATA METAANALYSIS; GENITAL-TRACT; ANTIBODY; INFECTION; PATHOLOGY; FERTILITY; SEROLOGY; SUSCEPTIBILITY; PREGNANCY; HISTORY;
D O I：
10.1093/femspd/ftx067
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
There is a need for more accurate Chlamydia trachomatis (CT) IgG antibody tests for tubal factor infertility (TFI) diagnostics. We evaluated the predictive value for TFI of Medac ELISA plus (MOMP) and multitarget Mikrogen ELISA (MOMP-CPAF-TARP). Based on Medac ELISA plus results, 183 subfertile women underwent either hysterosalpingography or laparoscopy to diagnose TFI. TFI was defined as extensive adhesions and/or distal occlusion of at least one tube. Women not fulfilling the definition of TFI served as controls. Serum was subsequently tested with Mikrogen ELISA and results were compared. 48 patients had TFI, 135 were controls. Mikrogen ELISA tested 125 patients positive/borderline of which 32% had TFI. Medac ELISA plus tested 77 patients positive/borderline of which 29.9% had TFI. Mikrogen tested 40 out of 48 TFI patients positive/borderline, Medac 23 out of 48. Kappa value was 0.34. PPV of Mikrogen ELISA and Medac ELISA plus were respectively 32% (95% CI 26%-39%) and 30% (95% CI 24%-37%), and NPV 86% (95% CI 81%-91%) and 76% (95% CI 70%-82%). Both tests were comparable in the prediction of TFI. However, Mikrogen ELISA had a higher NPV and might be more reliable in identifying patients without TFI. Kappa-value showed limited concordance between both tests.
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页数：7
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