Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

被引：7

作者：

Ilina, Polina ^{[1
]}

Ma, Xiaochu ^{[2
]}

Sintim, Herman O. ^{[2
]}

Tammela, Paivi ^{[1
]}

机构：

[1] Univ Helsinki, Fac Pharm, Drug Res Program, Div Pharmaceut Biosci, Helsinki, Finland

[2] Purdue Univ, Ctr Canc Res, Dept Chem, Inst Drug Discovery, W Lafayette, IN 47907 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 154卷

基金：

芬兰科学院;

关键词：

Autoinducer-2; Quorum sensing inhibitors; Bioreporter strain; Assay miniaturization; High throughput screening; AUTOINDUCER-2; PERSPECTIVES; INHIBITORS; AI-2; ART;

D O I：

10.1016/j.mimet.2018.10.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.

引用

页码：40 / 45

页数：6

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