Biocompatible glutathione capped gold clusters as one- and two-photon excitation fluorescence contrast agents for live cells imaging

被引：196

作者：

Polavarapu, Lakshminarayana ^{[1
]}

Manna, Manoj ^{[1
]}

Xu, Qing-Hua ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

来源：

NANOSCALE | 2011年 / 3卷 / 02期

关键词：

SEMICONDUCTOR QUANTUM DOTS; IN-VITRO; NANOPARTICLES; LUMINESCENCE; NANORODS; CYTOTOXICITY; ABSORPTION; CANCER; SIZE; NANOCLUSTERS;

D O I：

10.1039/c0nr00458h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The one- and two-photon excitation emission properties of water soluble glutathione monolayer protected gold clusters were investigated. Strong two-photon emission was observed from the gold clusters. The two-photon absorption cross section of these gold clusters in water was deduced from the z-scan measurement to be 189 740 GM, which is much higher compared to organic fluorescent dyes and quantum dots. These gold clusters also showed high photo-stability. The MTT assay showed that these gold clusters have low toxicity even at high concentrations. We have successfully demonstrated their applications for both one and two-photon excitation live cell imaging. The exceptional properties of these gold clusters make them a promising alternative for one- and two-photon bio-imaging and other nonlinear optical applications.

引用

页码：429 / 434

页数：6

共 59 条

[1] Thiolate ligands for synthesis of water-soluble gold clusters [J].

Ackerson, CJ ;

Jadzinsky, PD ;

Kornberg, RD .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (18) :6550-6551

[2] Silver Nanoparticles with Broad Multiband Linear Optical Absorption [J].

Bakr, Osman M. ;

Amendola, Vincenzo ;

Aikens, Christine M. ;

Wenseleers, Wim ;

Li, Rui ;

Dal Negro, Luca ;

Schatz, George C. ;

Stellacci, Francesco .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (32) :5921-5926

[3] Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy [J].

Bickford, Lissett ;

Sun, Jiantang ;

Fu, Kun ;

Lewinski, Nastassja ;

Nammalvar, Vengadesan ;

Chang, Joseph ;

Drezek, Rebekah .

NANOTECHNOLOGY, 2008, 19 (31)

[4] Semiconductor quantum dots and metal nanoparticles: syntheses, optical properties, and biological applications [J].

Biju, Vasudevanpillai ;

Itoh, Tamitake ;

Anas, Abdulaziz ;

Sujith, Athiyanathil ;

Ishikawa, Mitsuru .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (07) :2469-2495

[5] Antioxidants, oxidative damage and oxygen deprivation stress: a review [J].

Blokhina, O ;

Virolainen, E ;

Fagerstedt, KV .

ANNALS OF BOTANY, 2003, 91 (02) :179-194

[6] PHOTOINDUCED LUMINESCENCE FROM THE NOBLE-METALS AND ITS ENHANCEMENT ON ROUGHENED SURFACES [J].

BOYD, GT ;

YU, ZH ;

SHEN, YR .

PHYSICAL REVIEW B, 1986, 33 (12) :7923-7936

[7] Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates [J].

Chen, Cheng-Tai ;

Chen, Wei-Jen ;

Liu, Chao-Zong ;

Chang, Ling-Ya ;

Chen, Yu-Chie .

CHEMICAL COMMUNICATIONS, 2009, (48) :7515-7517

[8] Near-infrared fluorescent deoxyglucose analogue for tumor optical imaging in cell culture and living mice [J].

Cheng, Zhen ;

Levi, Jelena ;

Xiong, Zhengming ;

Gheysens, Olivier ;

Keren, Shay ;

Chen, Xiaoyuan ;

Gambhir, Sanjiv Sam .

BIOCONJUGATE CHEMISTRY, 2006, 17 (03) :662-669

[9] Facts and Artifacts in the Blinking Statistics of Semiconductor Nanocrystals [J].

Crouch, Catherine H. ;

Sauter, Orion ;

Wu, Xiaohua ;

Purcell, Robert ;

Querner, Claudia ;

Drndic, Marija ;

Pelton, Matthew .

NANO LETTERS, 2010, 10 (05) :1692-1698

[10]

Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334

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