Plasma proteome analysis in diet-induced obesity-prone and obesity-resistant rats

被引：37

作者：

Choi, Jung-Won ^{[1
]}

Wang, Xia ^{[1
]}

Joo, Jeong In ^{[1
]}

Kim, Dong Hyun ^{[1
]}

Oh, Tae Seok ^{[1
]}

Choi, Duk Kwon ^{[1
]}

Yun, Jong Won ^{[1
]}

机构：

[1] Daegu Univ, Dept Biotechnol, Kyungsan 712714, Kyungbuk, South Korea

来源：

PROTEOMICS | 2010年 / 10卷 / 24期

关键词：

Animal proteomics; 2-DE; High fat diet; Obesity-prone and -resistant rats; Plasma proteome; D-BINDING PROTEIN; RECEPTOR TYROSINE KINASE; ADIPOSE-TISSUE; ENERGY-BALANCE; WEIGHT-GAIN; BODY-WEIGHT; FOOD-INTAKE; GENE; ZINC-ALPHA-2-GLYCOPROTEIN; EXPRESSION;

D O I：

10.1002/pmic.201000391

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One of the major issues in the field of obesity is why some humans become obese and others resist development of obesity when exposed to high-calorie diets. Despite the same genetic background, namely obesity-prone (OP) and -resistant (OR) rats, differing responses have been demonstrated in a high fat diet-induced rodent model. The aim of the present study was to discover novel obesity-related biomarkers for susceptibility and/or resistance to obesity by proteomic analysis of OP and OR rat plasma. After feeding of high fat diet, OP rats gained approximately 25% more body weight than OR rats and were used for proteomic analysis using 2-DE combined with MALDI-TOF-MS. We categorized identified proteins into three groups by analysis of both average spot density in each group and individual spot density of six rats as a function of body weight. Consequently, category (1) included inter-a-inhibitor H4 heavy chain and fetuin B precursor, which can be used as novel plasma biomarkers for risk of obesity. Nine proteins of category (2) and (3) can also be plausible plasma markers in the study of obesity. This proteomic study is an important advancement over the previous steps needed for identification of OP and OR rats.

引用

页码：4386 / 4400

页数：15

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