Quantitative Analysis, Extraction Optimization, and Biological Evaluation of Cudrania tricuspidata Leaf and Fruit Extracts

被引:30
作者
Song, Seung-Hui [1 ]
Ki, Sung Hwan [2 ]
Park, Dae-Hun [3 ]
Moon, Hong-Seop [1 ]
Lee, Chang-Dai [4 ]
Yoon, In-Soo [5 ]
Cho, Seung-Sik [1 ]
机构
[1] Mokpo Natl Univ, Dept Pharm, Coll Pharm, Jeonnam 58554, South Korea
[2] Chosun Univ, Coll Pharm, Lab Toxicol, Gwangju 61452, South Korea
[3] Dongshin Univ, Dept Nursing, Naju Si 58245, Jeonnam, South Korea
[4] Mokpo Natl Univ, Dept Business Adm, Jeonnam 58554, South Korea
[5] Pusan Natl Univ, Dept Mfg Pharm, Coll Pharm, Busan 46241, South Korea
关键词
C. tricuspidata Bureau; simultaneous analysis; HPLC; xanthine oxidase; hyperuricemia; FLAVONOID-RICH EXTRACT; IN-VITRO; XANTHINE-OXIDASE; CHLOROGENIC ACID; CONSTITUENTS; ANTIOXIDANT; RUTIN; HYPERURICEMIA; INHIBITION; KAEMPFEROL;
D O I
10.3390/molecules22091489
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cudrania tricuspidata Bureau (Moraceae) shows numerous pharmacological effects and has been used in traditional herbal remedies for inflammation, gastritis, tumors, and liver diseases. However, no validated analytical method for the standardization and optimization of the biological properties of C. tricuspidata preparations has been reported. We developed and validated a reverse-phase high-performance liquid chromatography (HPLC) method for the separation and quantification of active markers. Ethanolic extracts of C. tricuspidata leaves were prepared and evaluated for chemical profiles and biological activities. The 80% ethanolic extract demonstrated the greatest antioxidant activity and phenolic content, while the 100% ethanolic extract had the greatest total flavonoid content and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed that chlorogenic acid, rutin, and kaempferol were present in C. tricuspidata leaf extracts. We postulated that the antioxidant and anti-hyperuricemic/gout effects of C. tricuspidata extract could be attributed to these marker compounds. Our results suggested that the flavonoid-rich fraction of the leaf extract may be utilized for the treatment and prevention of hyperuricemia-related diseases, and the validated method and marker compounds could be applied for the quality control of C. tricuspidata preparations.
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页数:14
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