The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients

被引：23

作者：

Deurenberg, RH ^{[1
]}

Nieuwenhuis, RF ^{[1
]}

Driessen, C ^{[1
]}

London, N ^{[1
]}

Stassen, FR ^{[1
]}

van Tiel, FH ^{[1
]}

Stobberingh, EE ^{[1
]}

Vink, C ^{[1
]}

机构：

[1] Univ Hosp Maastricht, Dept Med Microbiol, NL-6202 AZ Maastricht, Netherlands

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 245卷 / 01期

关键词：

toxic shock syndrome toxin-1; Staphylococcus aureus; real-time PCR; PFGE;

D O I：

10.1016/j.femsle.2005.03.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community-(CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p < 0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：185 / 189

页数：5

共 18 条

[1] Staphylococcus aureus express unique superantigens depending on the tissue source [J].

Banks, MC ;

Kamel, NS ;

Zabriskie, JB ;

Larone, DH ;

Ursea, D ;

Posnett, DN .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 (01) :77-86

[2] INTRAVENOUS IMMUNOGLOBULIN THERAPY FOR TOXIC SHOCK SYNDROME [J].

BARRY, W ;

HUDGINS, L ;

DONTA, ST ;

PESANTI, EL .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1992, 267 (24) :3315-3316

[3]

BERGERBACHI B, 1989, MOL GEN GENET, V219, P263

[4]

BLOMSTERHAUTAMAA DA, 1986, J BIOL CHEM, V261, P5783

[5] Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR [J].

Deurenberg, RH ;

Vink, C ;

Driessen, C ;

Bes, M ;

London, N ;

Etienne, J ;

Stobberingh, EE .

FEMS MICROBIOLOGY LETTERS, 2004, 240 (02) :225-228

[6] Exotoxins of Staphylococcus aureus [J].

Dinges, MM ;

Orwin, PM ;

Schlievert, PM .

CLINICAL MICROBIOLOGY REVIEWS, 2000, 13 (01) :16-+

[7] Superantigens - powerful modifiers of the immune system [J].

Fraser, J ;

Arcus, V ;

Kong, P ;

Baker, E ;

Proft, T .

MOLECULAR MEDICINE TODAY, 2000, 6 (03) :125-132

[8] Local variants of staphylococcal cassette chromosome mec in sporadic methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci:: Evidence of horizontal gene transfer? [J].

Hanssen, AM ;

Kjeldsen, G ;

Sollid, JUE .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :285-296

[9] Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (Alleles), and human disease [J].

Jarraud, S ;

Mougel, C ;

Thioulouse, J ;

Lina, G ;

Meugnier, H ;

Forey, F ;

Nesme, X ;

Etienne, J ;

Vandenesch, F .

INFECTION AND IMMUNITY, 2002, 70 (02) :631-641

[10] DETECTION OF GENES FOR ENTEROTOXINS, EXFOLIATIVE TOXINS, AND TOXIC SHOCK SYNDROME TOXIN-1 IN STAPHYLOCOCCUS-AUREUS BY THE POLYMERASE CHAIN-REACTION [J].

JOHNSON, WM ;

TYLER, SD ;

EWAN, EP ;

ASHTON, FE ;

POLLARD, DR ;

ROZEE, KR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (03) :426-430

← 1 2 →