Preparation of sialylated oligosaccharides employing recombinant trans-sialidase from Trypanosoma cruzi

被引：22
|
作者：
Neubacher, B
Schmidt, D
Ziegelmüller, P
Thiem, J
机构：
[1] Univ Hamburg, Inst Organ Chem, D-20146 Hamburg, Germany
[2] Univ Hamburg, Inst Biochem & Food Sci, D-20146 Hamburg, Germany
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2005年 / 3卷 / 08期
关键词：
D O I：
10.1039/b500042d
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Terminally sialylated oligosaccharides were synthesised employing recombinant trans-sialidase from Trypanosoma cruzi. Regio- and stereoselectively Sia-alpha(2-3)-Gal-beta R derivatives could be obtained in respectable yields, using combined chemical and enzymatic methodologies. An array of different disaccharide precursors such as Gal-beta(1-3)-GalNAc-alpha Ser/Thr, lactosides and lactosamide derivatives were sialylated and successfully purified by facile isolation procedures. Depending on the acceptor structure isolated, yields for trans-sialylation products were between 20 and 60%.
引用
收藏
页码：1551 / 1556
页数：6
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