Detection of resistance to rifampicin and decreased susceptibility to penicillin in Neisseria meningitidis by real-time multiplex polymerase chain reaction assay

被引：4
作者：
Stefanelli, Paola [1 ]
Neri, Arianna [1 ]
Carattoli, Alessandra [1 ]
Mastrantonio, Paola [1 ]
机构：
[1] Ist Super Sanita, Parasit & Immune Mediated Dis, Dept Infect, I-00161 Rome, Italy
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 58卷 / 02期
关键词：
Neisseria meningitidis; rifampicin; penicillin; real-time PCR;
D O I：
10.1016/j.diagmicrobio.2006.11.022
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
In this study, we designed primers and probes for the rpoB gene of Neisseria meningitidis to detect rifampicin-resistant strains in a combined use with primers and probes previously described for penicillin intermediate isolates. The assay was set up in the Light Cycler instrument using the Fluorescence Resonance Energy Transfer platform. The method was applied to N. meningitidis strains and to culture-negative cerebrospinal fluids (CSFs) from patients with meningococcal invasive disease. A spiked CSF was used to determine the sensitivity of the assay. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 244
页数：4
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