Identification of Thermophilic Bacterial Strains Producing Thermotolerant Hydrolytic Enzymes from Manure Compost

被引：30
作者：
Charbonneau, David M. [1 ]
Meddeb-Mouelhi, Fatma [1 ,2 ]
Boissinot, Maurice [3 ]
Sirois, Marc [1 ]
Beauregard, Marc [1 ]
机构：
[1] Univ Quebec Trois Rivieres, Dept Chim Biol, Trois Rivieres, PQ G9A 5H7, Canada
[2] Buckman Labs Canada, Vaudreuil Dorion, PQ J7V 5V5, Canada
[3] Univ Laval, Ctr Rech Infectiol, Ctr Hosp Univ Quebec, Ste Foy, PQ G1V 4G2, Canada
来源：
INDIAN JOURNAL OF MICROBIOLOGY | 2012年 / 52卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
Manure compost; Thermophilic bacteria; 16S rDNA; Hydrolytic enzymes; Thermal stability; PLATE ASSAY; GEN-NOV; BACILLUS; ENUMERATION; PROPOSAL;
D O I：
10.1007/s12088-011-0156-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65 degrees C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, alpha-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.
引用
收藏
页码：41 / 47
页数：7
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