Molecularly Imprinted Stationary Phase Prepared by Reverse Micro-Emulsion Polymerization for Selective Recognition of Gatifloxacin in Aqueous Media

被引：40

作者：

Zhang, Hui ^{[2
]}

Dramou, Pierre ^{[2
]}

He, Hua ^{[2
,3
]}

Tan, Shuhua ^{[1
]}

Chuong Pham-Huy ^{[4
]}

Pan, Hejian ^{[2
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

[2] China Pharmaceut Univ, Elementary Dept, Nanjing 210009, Peoples R China

[3] China Pharmaceut Univ, Minist Educ, Key Lab Drug Qual Control & Pharmacovigilance, Nanjing 210009, Peoples R China

[4] Univ Paris 05, Fac Pharm, F-75006 Paris, France

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2012年 / 50卷 / 06期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; BETA-CYCLODEXTRIN; MOBILE-PHASE; FUNCTIONAL MONOMER; POLYMERS; WATER; EXTRACTION; TRYPTOPHAN; SEPARATION; MICROSPHERES;

D O I：

10.1093/chromsci/bms028

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new stationary phase for selectively recognizing gatifloxacin in aqueous media based on molecularly imprinted microspheres (MIMs) has been prepared by water/oil reverse micro-emulsion polymerization. The MIMs were prepared using gatifloxacin as the template, N, N'-methylenebisacrylamide as cross-linker and acrylamide and acryloyl-β-CD (β-CD-A, synthesized by ester reaction of acrylic acid with β-CD) as combinatorial functional monomers. The surface morphology of MIMs was characterized by scanning electron microscopy. The properties of MIMs recognition for gatifloxacin in water were studied by adsorption kinetics, adsorption isotherms combined with Scatchard analysis and selective recognition experiments. The results showed that the synthesized MIMs had an excellent ability to selectively recognize gatifloxacin in aqueous media. MIMs were employed as the chromatographic stationary phase to successfully separate the template gatifloxacin from its analogues. Discovering the mechanism of the MIMs recognition revealed that the memory cavities in the surface of the MIMs and hydrophobic effects between the template and the cavities of the β-CD residues were the primary contributions to the special recognition process. © The Author [2012].

引用

页码：499 / 508

页数：10

共 37 条

[11] Computer-assisted design and synthesis of molecularly imprinted polymers for selective extraction of acetazolamide from human plasma prior to its voltammetric determination [J].

Khodadadian, Mehdi ;

Ahmadi, Farhad .

TALANTA, 2010, 81 (4-5) :1446-1453

[12] Direct Determination of the Enantiomeric Purity or Enantiomeric Composition of Methylpropionates Using a Single Capacitive Microsensor [J].

Kurzawski, Petra ;

Bogdanski, Anja ;

Schurig, Volker ;

Wimmer, Reinhard ;

Hierlemann, Andreas .

ANALYTICAL CHEMISTRY, 2009, 81 (05) :1969-1975

[13] Selective removal of 2,4-dichlorophenol from contaminated water using non-covalent imprinted microspheres [J].

Li, Ying ;

Li, Xin ;

Li, Yuqi ;

Qi, Jingyao ;

Bian, Jiang ;

Yuan, Yixing .

ENVIRONMENTAL POLLUTION, 2009, 157 (06) :1879-1885

[14] Uniformly sized β-cyclodextrin molecularly imprinted microspheres prepared by a novel surface imprinting technique for ursolic acid [J].

Liu, Hanmao ;

Liu, Chunhai ;

Yang, Xiaojing ;

Zeng, Songjun ;

Xiong, Yuanqin ;

Xu, Weijian .

ANALYTICA CHIMICA ACTA, 2008, 628 (01) :87-94

[15] Monolithic molecularly imprinted polymer for sulfamethoxazole and molecular recognition properties in aqueous mobile phase [J].

Liu, Xiangjun ;

Ouyan, Canbin ;

Zhao, Rui ;

Shangguan, Dihua ;

Chen, Yi ;

Liu, Guoquan .

ANALYTICA CHIMICA ACTA, 2006, 571 (02) :235-241

[16] Molecularly imprinted polymer beads: Suspension polymerization using a liquid perfluorocarbon as the dispersing phase [J].

Mayes, AG ;

Mosbach, K .

ANALYTICAL CHEMISTRY, 1996, 68 (21) :3769-3774

[17]

Pena E. B., 2009, ANAL BIOANAL CHEM, V393, P235

[18] Design of molecular imprinted polymers compatible with aqueous environment [J].

Piletska, Elena V. ;

Guerreiro, Antonio R. ;

Romero-Guerra, Maria ;

Chianella, Iva ;

Turner, Anthony P. F. ;

Piletsky, Sergey A. .

ANALYTICA CHIMICA ACTA, 2008, 607 (01) :54-60

[19] Molecularly imprinted polymers for solid phase extraction [J].

Qiao, Fengxia ;

Sun, Hanwen ;

Yan, Hongyuan ;

Row, Kyung Ho .

CHROMATOGRAPHIA, 2006, 64 (11-12) :625-634

[20] Molecularly imprinted polymer prepared with bonded β-cyclodextrin and acrylamide on functionalized silica gel for selective recognition of tryptophan in aqueous media [J].

Qin, Lei ;

He, Xi-Wen ;

Li, Wen-You ;

Zhang, Yu-Kui .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1187 (1-2) :94-102

← 1 2 3 4 →