17beta-estradiol induces both up-regulation and processing of cyclin E in a calpain-dependent manner in MCF-7 breast cancer cells

被引：18
|
作者：
Hou, Jianmei [1 ]
Wang, Xudong [1 ]
Li, Yang [1 ]
Liu, Xiaohong [1 ]
Wang, Zhuting [2 ]
An, Jing [1 ]
Yang, Li [1 ]
He, Yan [3 ]
机构：
[1] Guiyang Med Univ, Sch Basic Med, Canc Res Grp, Dept Physiol, Guiyang 550004, Guizhou, Peoples R China
[2] Guiyang Med Univ, Sch Basic Med, Dept Biochem, Guiyang 550004, Guizhou, Peoples R China
[3] Guiyang Med Univ, Prov Key Lab Mol Biochem, Guiyang 550004, Guizhou, Peoples R China
来源：
FEBS LETTERS | 2012年 / 586卷 / 06期
基金：
中国国家自然科学基金;
关键词：
17beta-estradiol; Cyclin E; Calpain; Proteolysis; Up-regulation; Breast cancer cell; MOLECULAR-WEIGHT FORMS; SIGNALING PATHWAY; ACTIVATION; SURVIVAL; ESTROGEN; TUMOR; EXPRESSION; CARCINOMA; ISOFORMS; CALCIUM;
D O I：
10.1016/j.febslet.2012.02.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the current study, we investigated whether 17beta-estradiol (E2) induces cyclin E expression and triggers cyclin E processing via calpain in MCF-7 breast cancer cells. We found that E2 induced increased expression of cyclin E in a slow and persistent manner, and a rapid yet sustained processing of cyclin E. In addition, estrogenic ethanol was able to stimulate cyclin E truncation. Calpeptin or ALLN greatly suppressed the E2-triggered cyclin E processing and its expression, suggesting a calpain-mediated action for E2. Finally, the E2-induced effects could also be significantly suppressed by BAPTA or U0126, indicating involvement of calcium/ERK signaling. Taken together, these results show that estrogen may contribute to both up-regulation and proteolysis of cyclin E through calpain in MCF-7 cells. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：892 / 896
页数：5
相关论文
共 50 条
  • [31] Up-regulation of HOXB cluster genes are epigenetically regulated in tamoxifen-resistant MCF7 breast cancer cells
    Yang, Seoyeon
    Lee, Ji-Yeon
    Hur, Ho
    Oh, Ji Hoon
    Kim, Myoung Hee
    BMB REPORTS, 2018, 51 (09) : 450 - 455
  • [32] Insights into significant pathways and gene interaction networks underlying breast cancer cell line MCF-7 treated with 17β-Estradiol (E2)
    Huan, Jinliang
    Wang, Lishan
    Xing, Li
    Qin, Xianju
    Feng, Lingbin
    Pan, Xiaofeng
    Zhu, Ling
    GENE, 2014, 533 (01) : 346 - 355
  • [33] Resveratrol induces apoptosis in breast cancer cells by E2F1-mediated up-regulation of ASPP1
    Shi, Yujie
    Yang, Shihe
    Troup, Sandi
    Lu, Xin
    Callaghan, Steve
    Park, David S.
    Xing, Ying
    Yang, Xiaohe
    ONCOLOGY REPORTS, 2011, 25 (06) : 1713 - 1719
  • [34] Ethiadin Induces Apoptosis and Suppresses Growth of MCF-7 Breast Cancer Cells by Regulating the Phosphorylation of Glycogen Synthase Kinase 3 Beta (GSK3β)
    Ren, Yazhe
    Bao, Guangsen
    Yang, Hengbin
    Cao, Zhangjun
    Shao, Zhiyu
    Zhang, Yunlong
    DISCOVERY MEDICINE, 2022, 33 (169) : 55 - 67
  • [35] The actin filament cross-linker L-plastin confers resistance to TNF-α in MCF-7 breast cancer cells in a phosphorylation-dependent manner
    Janji, Bassam
    Vallar, Laurent
    Al Tanoury, Ziad
    Bernardin, Francois
    Vetter, Guillaume
    Schaffner-Reckinger, Elisabeth
    Berchem, Guy
    Friederich, Evelyne
    Chouaib, Salem
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2010, 14 (06) : 1264 - 1275
  • [36] Insulin-like growth factor-1 and 17β-estradiol down-regulate prostate apoptosis response-4 expression in MCF-7 breast cancer cells
    Casolari, Debora A.
    Pereira, Michelly C.
    De Bessa Garcia, Simone A.
    Nagai, Maria A.
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2011, 28 (03) : 337 - 342
  • [37] Expression and regulation of WNT10B in human cancer:: Up-regulation of WNT10B in MCF-7 cells by β-estradiol and down-regulation of WNT10B in NT2 cells by retinoic acid
    Kirikoshi, H
    Katoh, M
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2002, 10 (04) : 507 - 511
  • [38] Paroxetine Induces Apoptosis of Human Breast Cancer MCF-7 Cells through Ca2+-and p38 MAP Kinase-Dependent ROS Generation
    Cho, Young-Woo
    Kim, Eun-Jin
    Nyiramana, Marie Merci
    Shin, Eui-Jung
    Jin, Hana
    Ryu, Ji Hyeon
    Kang, Kee Ryeon
    Lee, Gyeong-Won
    Kim, Hye Jung
    Han, Jaehee
    Kang, Dawon
    CANCERS, 2019, 11 (01):
  • [39] ERβ-dependent neuroglobin up-regulation impairs 17β-estradiol-induced apoptosis in DLD-1 colon cancer cells upon oxidative stress injury
    Fiocchetti, Marco
    Camilli, Giulia
    Acconcia, Filippo
    Leone, Stefano
    Ascenzi, Paolo
    Marino, Maria
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2015, 149 : 128 - 137
  • [40] Surfactin Induces Apoptosis and G2/M Arrest in Human Breast Cancer MCF-7 Cells Through Cell Cycle Factor Regulation
    Cao, Xiaohong
    Wang, A. H.
    Jiao, R. Z.
    Wang, C. L.
    Mao, D. Z.
    Yan, L.
    Zeng, B.
    CELL BIOCHEMISTRY AND BIOPHYSICS, 2009, 55 (03) : 163 - 171
12345