Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells

被引：304
作者：
Heiskanen, KM
Bhat, MB
Wang, HW
Ma, JJ
Nieminen, AL
机构：
[1] Case Western Reserve Univ, Sch Med, Dept Anat, Cleveland, OH 44106 USA
[2] Case Western Reserve Univ, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44106 USA
[3] Case Western Reserve Univ, Sch Med, Dept Biomed Engn, Cleveland, OH 44106 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 09期
关键词：
D O I：
10.1074/jbc.274.9.5654
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cytochrome c is released from mitochondria into the cytosol in cells undergoing apoptosis. The temporal relationship between cytochrome c release and loss of mitochondrial membrane potential was monitored by laser-scanning confocal microscopy in single living pheochromocytoma-6 cells undergoing apoptosis induced by staurosporine. Mitochondrial membrane potential monitored by tetramethylrhodamine methyl ester decreased abruptly in individual cells from 2 to 7 h after treatment with staurosporine. Depolarization was accompanied by cytochrome c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.
引用
收藏
页码：5654 / 5658
页数：5
相关论文
共 31 条
[1]   RECENT PROGRESS ON REGULATION OF THE MITOCHONDRIAL PERMEABILITY TRANSITION PORE - A CYCLOSPORINE-SENSITIVE PORE IN THE INNER MITOCHONDRIAL-MEMBRANE [J].
BERNARDI, P ;
BROEKEMEIER, KM ;
PFEIFFER, DR .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1994, 26 (05) :509-517
[2]   Characterization of the cell death process induced by staurosporine in human neuroblastoma cell lines [J].
Boix, J ;
Llecha, N ;
Yuste, VJ ;
Comella, JX .
NEUROPHARMACOLOGY, 1997, 36 (06) :811-821
[3]   Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization [J].
Bossy-Wetzel, E ;
Newmeyer, DD ;
Green, DR .
EMBO JOURNAL, 1998, 17 (01) :37-49
[4]   Mitochondrial control of apoptosis:: the role of cytochrome c [J].
Cai, JY ;
Yang, J ;
Jones, DP .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1366 (1-2) :139-149
[5]   MEMBRANE-POTENTIAL CAN BE DETERMINED IN INDIVIDUAL CELLS FROM THE NERNSTIAN DISTRIBUTION OF CATIONIC DYES [J].
EHRENBERG, B ;
MONTANA, V ;
WEI, MD ;
WUSKELL, JP ;
LOEW, LM .
BIOPHYSICAL JOURNAL, 1988, 53 (05) :785-794
[6]   RHODAMINE-123 AS A PROBE OF TRANSMEMBRANE POTENTIAL IN ISOLATED RAT-LIVER MITOCHONDRIA - SPECTRAL AND METABOLIC PROPERTIES [J].
EMAUS, RK ;
GRUNWALD, R ;
LEMASTERS, JJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 850 (03) :436-448
[7]   Mitochondria and apoptosis [J].
Green, DR ;
Reed, JC .
SCIENCE, 1998, 281 (5381) :1309-1312
[8]   Imaging the permeability pore transition in single mitochondria [J].
Hüser, J ;
Rechenmacher, CE ;
Blatter, LA .
BIOPHYSICAL JOURNAL, 1998, 74 (04) :2129-2137
[9]   From calcium signaling to cell death: two conformations for the mitochondrial permeability transition pore. Switching from low- to high-conductance state [J].
Ichas, F ;
Mazat, JP .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1366 (1-2) :33-50
[10]   The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis [J].
Kluck, RM ;
BossyWetzel, E ;
Green, DR ;
Newmeyer, DD .
SCIENCE, 1997, 275 (5303) :1132-1136
1234