CRISPR/Cas9 Screening for Identification of Genes Required for the Growth of Ovarian Clear Cell Carcinoma Cells

被引：0

作者：

Kawabata, Ayako ^{[1
,2
]}

Hayashi, Tomoatsu ^{[1
]}

Akasu-Nagayoshi, Yoko ^{[1
,2
]}

Yamada, Ai ^{[1
]}

Shimizu, Naomi ^{[1
]}

Yokota, Naoko ^{[3
]}

Nakato, Ryuichiro ^{[3
]}

Shirahige, Katsuhiko ^{[4
]}

Okamoto, Aikou ^{[2
]}

Akiyama, Tetsu ^{[1
]}

机构：

[1] Univ Tokyo, Inst Quantitat Biosci, Lab Mol & Genet Informat, Tokyo 1130032, Japan

[2] Jikei Univ, Dept Obstet & Gynecol, Sch Med, Tokyo 1058461, Japan

[3] Univ Tokyo, Inst Quantitat Biosci, Lab Computat Genet, Tokyo 1130032, Japan

[4] Univ Tokyo, Inst Quantitat Biosci, Lab Genome Struct & Funct, Tokyo 1130032, Japan

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2022年 / 44卷 / 04期

基金：

日本学术振兴会;

关键词：

ovarian cancer; ovarian clear cell carcinoma; CRISPR; Cas9; system; proliferation; tumorigenesis; POOR-PROGNOSIS; CANCER; MUTATIONS; ARID1A;

D O I：

10.3390/cimb44040108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epithelial ovarian cancer is classified into four major histological subtypes: serous, clear cell, endometrioid and mucinous. Ovarian clear cell carcinoma (OCCC) responds poorly to conventional chemotherapies and shows poor prognosis. Thus, there is a need to develop new drugs for the treatment of OCCC. In this study, we performed CRISPR/Cas9 screens against OCCC cell lines and identified candidate genes important for their proliferation. We found that quite different genes are required for the growth of ARID1A and PIK3CA mutant and wild-type OCCC cell lines, respectively. Furthermore, we found that the epigenetic regulator KDM2A and the translation regulator PAIP1 may play important roles in the growth of ARID1A and PIK3CA mutant, but not wild-type, OCCC cells. The results of our CRISPR/Cas9 screening may be useful in elucidating the molecular mechanism of OCCC tumorigenesis and in developing OCCC-targeted drugs.

引用

页码：1587 / 1596

页数：10

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