CRISPR/Cas9 Screening for Identification of Genes Required for the Growth of Ovarian Clear Cell Carcinoma Cells

被引：0
作者：
Kawabata, Ayako [1 ,2 ]
Hayashi, Tomoatsu [1 ]
Akasu-Nagayoshi, Yoko [1 ,2 ]
Yamada, Ai [1 ]
Shimizu, Naomi [1 ]
Yokota, Naoko [3 ]
Nakato, Ryuichiro [3 ]
Shirahige, Katsuhiko [4 ]
Okamoto, Aikou [2 ]
Akiyama, Tetsu [1 ]
机构：
[1] Univ Tokyo, Inst Quantitat Biosci, Lab Mol & Genet Informat, Tokyo 1130032, Japan
[2] Jikei Univ, Dept Obstet & Gynecol, Sch Med, Tokyo 1058461, Japan
[3] Univ Tokyo, Inst Quantitat Biosci, Lab Computat Genet, Tokyo 1130032, Japan
[4] Univ Tokyo, Inst Quantitat Biosci, Lab Genome Struct & Funct, Tokyo 1130032, Japan
来源：
CURRENT ISSUES IN MOLECULAR BIOLOGY | 2022年 / 44卷 / 04期
基金：
日本学术振兴会;
关键词：
ovarian cancer; ovarian clear cell carcinoma; CRISPR; Cas9; system; proliferation; tumorigenesis; POOR-PROGNOSIS; CANCER; MUTATIONS; ARID1A;
D O I：
10.3390/cimb44040108
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Epithelial ovarian cancer is classified into four major histological subtypes: serous, clear cell, endometrioid and mucinous. Ovarian clear cell carcinoma (OCCC) responds poorly to conventional chemotherapies and shows poor prognosis. Thus, there is a need to develop new drugs for the treatment of OCCC. In this study, we performed CRISPR/Cas9 screens against OCCC cell lines and identified candidate genes important for their proliferation. We found that quite different genes are required for the growth of ARID1A and PIK3CA mutant and wild-type OCCC cell lines, respectively. Furthermore, we found that the epigenetic regulator KDM2A and the translation regulator PAIP1 may play important roles in the growth of ARID1A and PIK3CA mutant, but not wild-type, OCCC cells. The results of our CRISPR/Cas9 screening may be useful in elucidating the molecular mechanism of OCCC tumorigenesis and in developing OCCC-targeted drugs.
引用
收藏
页码：1587 / 1596
页数：10
相关论文
共 41 条
  • [1] Anglesio MS, 2013, PLOS ONE, V8, DOI [10.1371/annotation/ffcaf179-872f-470b-8bb6-f06d8ba6d03a, 10.1371/journal.pone.0072162]
  • [2] Upregulation of PAIP1 promotes the gallbladder tumorigenesis through regulating PLK1 level
    Bi, Jianping
    Ma, Hong
    Liu, Yafei
    Huang, Ai
    Xiao, Yong
    Shu, Wen-Jie
    Du, Haining
    Zhang, Tao
    [J]. ANNALS OF TRANSLATIONAL MEDICINE, 2021, 9 (12)
  • [3] ARID1A mutant ovarian clear cell carcinoma: A clear target for synthetic lethal strategies
    Caumanns, Joseph J.
    Wisman, G. Bea A.
    Berns, Katrien
    van der Zee, Ate G. J.
    de Jong, Steven
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2018, 1870 (02): : 176 - 184
  • [4] Integrative Kinome Profiling Identifies mTORC1/2 Inhibition as Treatment Strategy in Ovarian Clear Cell Carcinoma
    Caumanns, Joseph J.
    Berns, Katrien
    Wisman, G. Bea A.
    Fehrmann, Rudolf S. N.
    Tomar, Tushar
    Klip, Harry
    Meersma, Gert J.
    Hijmans, E. Marielle
    Gennissen, Annemiek M. C.
    Duiker, Evelien W.
    Weening, Desiree
    Itamochi, Hiroaki
    Kluin, Roelof J. C.
    Reyners, Anna K. L.
    Birrer, Michael J.
    Salvesen, Helga B.
    Vergote, Ignace
    van Nieuwenhuysen, Els
    Brenton, James
    Braicu, E. Ioana
    Kupryjanczyk, Jolanta
    Spiewankiewicz, Beata
    Mittempergher, Lorenza
    Bernards, Rene
    van der Zee, Ate G. J.
    de Jong, Steven
    [J]. CLINICAL CANCER RESEARCH, 2018, 24 (16) : 3928 - 3940
  • [5] Lysine demethylase 2A promotes stemness and angiogenesis of breast cancer by upregulating Jagged1
    Chen, Jing-Yi
    Li, Chien-Feng
    Chu, Pei-Yi
    Lai, You-Syuan
    Chen, Chung-Hsing
    Jiang, Shih Sheng
    Hou, Ming-Feng
    Hung, Wen-Chun
    [J]. ONCOTARGET, 2016, 7 (19) : 27689 - 27710
  • [6] Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer
    Cheung, Hiu Wing
    Cowley, Glenn S.
    Weir, Barbara A.
    Boehm, Jesse S.
    Rusin, Scott
    Scott, Justine A.
    East, Alexandra
    Ali, Levi D.
    Lizotte, Patrick H.
    Wong, Terence C.
    Jiang, Guozhi
    Hsiao, Jessica
    Mermel, Craig H.
    Getz, Gad
    Barretina, Jordi
    Gopal, Shuba
    Tamayo, Pablo
    Gould, Joshua
    Tsherniak, Aviad
    Stransky, Nicolas
    Luo, Biao
    Ren, Yin
    Drapkin, Ronny
    Bhatia, Sangeeta N.
    Mesirov, Jill P.
    Garraway, Levi A.
    Meyerson, Matthew
    Lander, Eric S.
    Root, David E.
    Hahn, William C.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (30) : 12372 - 12377
  • [7] Lack of effective systemic therapy for recurrent clear cell carcinoma of the ovary
    Crotzer, David R.
    Sun, Charlotte C.
    Coleman, Robert L.
    Wolf, Judith K.
    Levenback, Charles F.
    Gershenson, David M.
    [J]. GYNECOLOGIC ONCOLOGY, 2007, 105 (02) : 404 - 408
  • [8] Devor JRL Eric J., 2021, Obstet Gynecol Res, V4, P233
  • [9] A role for PAX8 in the tumorigenic phenotype of ovarian cancer cells
    Di Palma, Tina
    Lucci, Valeria
    de Cristofaro, Tiziana
    Filippone, Maria Grazia
    Zannini, Mariastella
    [J]. BMC CANCER, 2014, 14
  • [10] Next-generation characterization of the Cancer Cell Line Encyclopedia
    Ghandi, Mahmoud
    Huang, Franklin W.
    Jane-Valbuena, Judit
    Kryukov, Gregory V.
    Lo, Christopher C.
    McDonald, E. Robert, III
    Barretina, Jordi
    Gelfand, Ellen T.
    Bielski, Craig M.
    Li, Haoxin
    Hu, Kevin
    Andreev-Drakhlin, Alexander Y.
    Kim, Jaegil
    Hess, Julian M.
    Haas, Brian J.
    Aguet, Francois
    Weir, Barbara A.
    Rothberg, Michael V.
    Paolella, Brenton R.
    Lawrence, Michael S.
    Akbani, Rehan
    Lu, Yiling
    Tiv, Hong L.
    Gokhale, Prafulla C.
    De Weck, Antoine
    Mansour, Ali Amin
    Oh, Coyin
    Shih, Juliann
    Hadi, Kevin
    Rosen, Yanay
    Bistline, Jonathan
    Venkatesan, Kavitha
    Reddy, Anupama
    Sonkin, Dmitriy
    Liu, Manway
    Lehar, Joseph
    Korn, Joshua M.
    Porter, Dale A.
    Jones, Michael D.
    Golji, Javad
    Caponigro, Giordano
    Taylor, Jordan E.
    Dunning, Caitlin M.
    Creech, Amanda L.
    Warren, Allison C.
    McFarland, James M.
    Zamanighomi, Mahdi
    Kauffmann, Audrey
    Stransky, Nicolas
    Imielinski, Marcin
    [J]. NATURE, 2019, 569 (7757) : 503 - +
12345