High speed, inverted optical-resolution photoacoustic microscopy

被引：1

作者：

Rao, Bin ^{[1
]}

Maslov, Konstantin ^{[1
]}

Danielli, Amos ^{[1
]}

Chen, Ruiming ^{[2
]}

Shung, K. Kirk ^{[2
]}

Zhou, Qifa ^{[2
]}

Wang, Lihong V. ^{[1
]}

机构：

[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, Campus Box 1097,1 Brookings Dr, St Louis, MO 63130 USA

[2] Univ Southern Calif, Dept Biomed Engn, Los Angeles, CA 90089 USA

来源：

PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2011 | 2011年 / 7899卷

基金：

美国国家卫生研究院;

关键词：

FLOW;

D O I：

10.1117/12.873568

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Photoacoustic microscopy (PAM) offers label-free, optical absorption contrast. A high-speed, high-resolution PAM system in an inverted microscope configuration with a laser pulse repetition rate of 100,000 Hz and a stationary ultrasonic transducer was built. Four-dimensional in vivo imaging of microcirculation in mouse skin was achieved at 18 three-dimensional volumes per second with repeated two-dimensional raster scans of 100 by 50 points. The corresponding two-dimensional B-scan (50 A-lines) frame rate was 1800 Hz, and the one-dimensional A-scan rate was 90,000 Hz. The lateral resolution is 0.23+/-0.03 mu m for Au nano-wire imaging, which is 2.0 times below the diffraction limit.

引用

页数：7

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