Tumor suppressor gene hypermethylation as a predictor of gastric stromal tumor behavior

被引:80
作者
House, MG
Guo, MZ
Efron, DT
Lillemoe, KD
Cameron, JL
Syphard, JE
Hooker, CM
Abraham, SC
Montgomery, EA
Herman, JG
Brock, MV
机构
[1] Johns Hopkins Med Inst, Dept Surg, Baltimore, MD 21205 USA
[2] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
[3] Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Div Tumor Biol, Dept Oncol, Baltimore, MD 21231 USA
关键词
methylation; GIST; tumor suppressor genes;
D O I
10.1016/j.gassur.2003.08.002
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The growing understanding of the epigenetic changes associated with cancer, including aberrant promoter methylation of tumor suppressor genes that afford selective growth advantages to human neoplasms, suggests that the characterization of gene methylation patterns among gastrointestinal stromal tumors (GISTs) may be useful for predicting tumor behavior. Thirty-eight c-kit-positive gastric stromal tumors were subjected to methylation-specific polymerase chain reaction (MSP) to detect promoter methylation associated with I I candidate tumor suppressor genes (p16/INK4a, APC, MGMT, hMLH1, p73, E-cadherin, RAR-beta, RASSF1A, RB, ER, and DAPK), established to have a role in tumorigenesis of several solid human organs. Aberrant methylation of any of the I I candidate tumor suppressor genes was detected in 84% of all GISTs. In decreasing order of frequency, the six most commonly methylated genes were: MGMT (47%), p16 (45%), RASSF1A (40%), E-cadherin (37%), hMLH1 (34%), and APC (31%). For all of the GISTs, promoter methylation was less reliable than tumor mitotic rate in predicting 5-year tumor-free survival for the GISTs; however, E-cadherin methylation was a multivariate prognostic factor for early recurrence of GISTs (50% at 2 years; P = 0.030). Among the mitotically active (>5 per 50 high-power field), histologically indistinguishable GISTs, E-cadherin methylation was an independent predictor of tumor-related mortality: 5-year disease-free survival was worse for the E-cadherin methylated GISTs (19%) compared to the E-cadherin unmethylated tumors (71%; P = 0.010). Detection of methylation within selected genes may afford a reliable and accurate molecular marker system for predicting neoplastic behavior among GISTs. This study supports the methylation status of E-cadherin as a prognostic marker for early GIST recurrence and survival. (C) 2003 The Society for Surgery of the Alimentary Tract.
引用
收藏
页码:1004 / 1013
页数:10
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