A real time metabolomic profiling approach to detecting fish fraud using rapid evaporative ionisation mass spectrometry

被引:86
作者
Black, Connor [1 ]
Chevallier, Olivier P. [1 ]
Haughey, Simon A. [1 ]
Balog, Julia [2 ,3 ]
Stead, Sara [4 ]
Pringle, Steven D. [4 ]
Riina, Maria V. [5 ]
Martucci, Francesca [5 ]
Acutis, Pier L. [5 ]
Morris, Mike [4 ]
Nikolopoulos, Dimitrios S. [6 ]
Takats, Zoltan [3 ]
Elliott, Christopher T. [1 ]
机构
[1] Queens Univ Belfast, Inst Global Food Secur, Adv ASSET Ctr, Sch Biol Sci, 18-30 Malone Rd, Belfast BT9 5BN, Antrim, North Ireland
[2] Waters Res Ctr, 7 Zahony St, H-1031 Budapest, Hungary
[3] Imperial Coll London, Sout Kensington Campus,Sir Alexander Fleming Bldg, London SW7 2AZ, England
[4] Waters Corp, Altrincham Rd, Wilmslow SK9 4AX, Cheshire, England
[5] Ist Zooprofilatt Sperimentale Piemonte Liguria &, Via Bologna 148, I-10154 Turin, Italy
[6] Queens Univ Belfast, Sch Elect Elect Engn & Comp Sci, Belfast, Antrim, North Ireland
基金
英国生物技术与生命科学研究理事会;
关键词
REIMS; Real time; No sample preparation; Fish; Species identification; Catch method; DNA BARCODING REVEALS; MEAT-PRODUCTS; FOOD FRAUD; SEAFOOD; IDENTIFICATION; AUTHENTICATION; SUBSTITUTION; MARKET; QUALITY; RATES;
D O I
10.1007/s11306-017-1291-y
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Fish fraud detection is mainly carried out using a genomic profiling approach requiring long and complex sample preparations and assay running times. Rapid evaporative ionisation mass spectrometry (REIMS) can circumvent these issues without sacrificing a loss in the quality of results. To demonstrate that REIMS can be used as a fast profiling technique capable of achieving accurate species identification without the need for any sample preparation. Additionally, we wanted to demonstrate that other aspects of fish fraud other than speciation are detectable using REIMS. 478 samples of five different white fish species were subjected to REIMS analysis using an electrosurgical knife. Each sample was cut 8-12 times with each one lasting 3-5 s and chemometric models were generated based on the mass range m/z 600-950 of each sample. The identification of 99 validation samples provided a 98.99% correct classification in which species identification was obtained near-instantaneously (ae 2 s) unlike any other form of food fraud analysis. Significant time comparisons between REIMS and polymerase chain reaction (PCR) were observed when analysing 6 mislabelled samples demonstrating how REIMS can be used as a complimentary technique to detect fish fraud. Additionally, we have demonstrated that the catch method of fish products is capable of detection using REIMS, a concept never previously reported. REIMS has been proven to be an innovative technique to help aid the detection of fish fraud and has the potential to be utilised by fisheries to conduct their own quality control (QC) checks for fast accurate results.
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页数:13
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