The purpose of this study was to investigate the role of warm-up intensity on anaerobic performance. The research material included 18 basketball players with an average age of 24,3 +/- 3,1 years, body mass and height respectively 86,2 +/- 6,9 kg and 193,3 +/- 4,5 cm. All of the players performed anaerobic running test 10 x 30 m with a 20 s rest interval between consecutive sprints. The anaerobic test was conducted twice following two types of warm-up procedures: aerobic and anaerobic. Blood samples were drawn from the fingertip at rest, after the warm-up and in the 4(th) and 15(th) min of recovery to evaluate plasma lactate concentration. The results indicate a statistically significant effect (ANOVA, p< 0, 05) of the type of warm-up procedures on results of the anaerobic 10 x 30 m test as well as particular 30 m sprints (p<0,001). The total post exercise results of the 10 x 30 m test trial after anaerobic warm-up was statistically significant (p< 0,05) lowered versus the aerobic warm-up procedures. The intensity of the warm-tip significantly (p<0,0001) effected the blood plasma lactate concentration. Statistically analysis showed a significant differences (p<0,05) in post exercise lactate concentration after the two types of warm-up procedures. In conclusion, the results of this study indicate, that warm-up with high intensity performance might be beneficial in anaerobic competition.