Biomimetic electrical stimulation induces rat bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells via TGF-beta 1 in vitro

被引：15
作者：
He, Xueling [1 ,2 ]
Li, Liang [1 ]
Tang, Min [1 ]
Zeng, Ye [1 ]
Li, Huiming [1 ]
Yu, Xiaoqin [1 ]
机构：
[1] Sichuan Univ, West China Sch Basic Med Sci & Forens Med, Inst Biomed Engn, Chengdu 610041, Peoples R China
[2] Sichuan Univ, Lab Anim Ctr, Chengdu 610041, Peoples R China
来源：
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2019年 / 148卷
关键词：
Electrical stimulation; Bone marrow-derived mesenchymal stem cells; Cardiomyogenic differentiation; Transforming growth factor-beta 1; CARDIAC DIFFERENTIATION; TRANSPLANTATION; REPAIR; ELECTROSTIMULATION; IMPLANTATION; MYOCARDIUM; THERAPY; MATRIX;
D O I：
10.1016/j.pbiomolbio.2017.09.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Electrical conductance is one of the factors of the microenvironment of cardiomyocytes, and electrical stimulation (ES) has been shown to modulate the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) toward a cardiomyogenic fate. Transforming growth factor-beta 1 (TGF-beta 1) stimulates the cardiomyogenic marker expression in BMSCs. Herein, we promoted the differentiation of BMSCs into cardiomyocyte-like cells using ES to confirm if TGF-beta 1 mediates this event in vitro. ES increased protein levels of TGF-beta 1 in BMSCs, and this effect was better than that observed with 5-azacytidine (5-Aza). The effect of ES on promoting cardiomyogenic marker expression in BMSCs was enhanced by TGF-beta 1. Furthermore, the protein expression levels of Connexin43 (Cx43) and Alpha-actinin 2 (ACTN2) induced by ES in BMSCs were significantly decreased by pirfenidone. These results show that ES promotes cardiomyocyte-like cells differentiation in rat BMSCs and is possibly mediated by TGF-beta 1 in vitro. (C) 2017 Published by Elsevier Ltd.
引用
收藏
页码：47 / 53
页数：7
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