Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D

被引：8

作者：

Chakritbudsabong, Warunya ^{[1
]}

Sariya, Ladawan ^{[2
]}

Pamonsupornvichit, Sirikron ^{[2
]}

Pronarkngver, Rassmeepen ^{[2
]}

Chaiwattanarungruengpaisan, Somjit ^{[2
]}

Ferreira, Joao N. ^{[3
]}

Setthawong, Piyathip ^{[4
]}

Phakdeedindan, Praopilas ^{[5
]}

Techakumphu, Mongkol ^{[4
]}

Tharasanit, Theerawat ^{[4
]}

Rungarunlert, Sasitorn ^{[6
]}

机构：

[1] Mahidol Univ, Dept Clin Sci & Publ Hlth, Fac Vet Sci, Salaya 73170, Nakhon Pathom, Thailand

[2] Mahidol Univ, Monitoring & Surveillance Ctr Zoonot Dis Wildlife, Fac Vet Sci, Salaya 73170, Nakhon Pathom, Thailand

[3] Natl Univ Singapore, Dept Oral & Maxillofacial Surg, Fac Dent, 11 Lower Kent Ridge Rd, Singapore 119083, Singapore

[4] Chulalongkorn Univ, Fac Vet Sci, Dept Obstet Gynaecol & Reprod, Bangkok 10330, Thailand

[5] Chulalongkorn Univ, Dept Physiol, Biochem Unit, Fac Vet Sci, Bangkok 10330, Thailand

[6] Mahidol Univ, Fac Vet Sci, Dept Preclin & Appl Anim Sci, Salaya 73170, Nakhon Pathom, Thailand

来源：

STEM CELL RESEARCH | 2017年 / 24卷

关键词：

D O I：

10.1016/j.scr.2017.08.005

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T. (C) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license

引用

页码：21 / 24

页数：4

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