The Effect of Tocotrienol-Rich Fraction on the Expression of Glutathione S-Transferase Isoenzymes in Mice Liver

被引:5
作者
Atla, Ahmed [1 ,2 ]
Alrawaiq, Nadia Salem [1 ]
Abdullah, Azman [1 ]
机构
[1] Univ Kebangsaan Malaysia, Fac Med, Dept Pharmacol, Jalan Yaacob Latif, Kuala Lumpur 56000, Federal Territo, Malaysia
[2] Tripoli Univ, Fac Med Technol, Dept Anesthesia & Intens Care, Tripoli, Libya
来源
SAINS MALAYSIANA | 2018年 / 47卷 / 11期
关键词
Glutathione S-transferase; isoenzymes; liver; tocotrienols; TRF; MARKER ENZYME-ACTIVITIES; GENE-EXPRESSION; VITAMIN-E; GAMMA-TOCOTRIENOL; OXIDATIVE STRESS; ALPHA-TOCOPHEROL; PALM OIL; CANCER; RATS; SUPPRESSION;
D O I
10.17576/jsm-2018-4711-23
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Glutathione S-trans erase isoenzymes (GSTs) catalyze the conjugation reaction between glutathione and electrophilic compounds. GSTs are involved in the detoxification of toxic and carcinogenic compounds, thus protecting the body from toxic injuries. Tocotrienols are part of the vitamin E family and is believed to possess potent antioxidant activity. The objective of this study was to determine the effect of increasing doses of tocotrienol rich fraction (TRF) supplementation on liver GSTs gene and protein expression. A total of 30 male ICR white mice were divided into five groups (n=6 for each group) and given treatment for 14 days through oral supplementation. Groups were divided as follows: - three groups administered with TRF at doses of 200, 500 and 1000 mg/kg, respectively, a positive control group administered with 100 mg/kg butylated hydroxyanisole (BHA) and a control group administered with only the vehicle (corn oil). At day 15, the mice were sacrificed and their livers isolated. Total RNA was extracted from the liver and quantitative real-time polymerase chain reaction (qPCR) assays were performed to analyze GSTs gene expression. Total liver protein was also extracted and the protein expression of GSTs was determined by Western blotting. The results showed that TRF oral supplementation caused a significant dose-dependent increase in liver GST isoenzymes gene and protein expression, compared to controls. In conclusion, TRF oral supplementation for 14 days resulted in increased gene and protein expression of GST isoenzymes in mice liver dose-dependently, with the highest expression seen in mice treated with 1000 mg/kg TRF.
引用
收藏
页码:2799 / 2809
页数:11
相关论文
共 60 条
[1]  
Aan GoonJo Aan GoonJo, 2015, International Journal of Advanced Biological and Biomedical Research, V3, P222
[2]   Analysis of the role of Nrf2 in the expression of liver proteins in mice using two-dimensional gel-based proteomics [J].
Abdullah, Azman ;
Kitteringham, Neil R. ;
Jenkins, Rosalind E. ;
Goldring, Christopher ;
Higgins, Larry ;
Yamamoto, Masayuki ;
Hayes, John ;
Park, B. Kevin .
PHARMACOLOGICAL REPORTS, 2012, 64 (03) :680-697
[3]   Nitrofurantoin-induced hepatic and pulmonary biochemical changes in mice fed different vitamin E doses [J].
Adam, A ;
Marzuki, A ;
Ngah, WZW ;
Top, GM .
PHARMACOLOGY & TOXICOLOGY, 1996, 79 (06) :334-339
[4]  
Adaramoye Oluwatosin Adekunle, 2012, Journal of Basic and Clinical Physiology and Pharmacology, V23, P69, DOI 10.1515/jbcpp.2011.0042
[5]   Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status [J].
Ajith, T. A. ;
Hema, U. ;
Aswathy, M. S. .
FOOD AND CHEMICAL TOXICOLOGY, 2007, 45 (11) :2267-2272
[6]   Emerging role of Nrf2 in protecting against hepatic and gastrointestinal disease [J].
Aleksunes, Lauren M. ;
Manautou, Jose E. .
TOXICOLOGIC PATHOLOGY, 2007, 35 (04) :459-473
[7]   Drug Metabolism in the Liver [J].
Almazroo, Omar Abdulhameed ;
Miah, Mohammad Kowser ;
Venkataramanan, Raman .
CLINICS IN LIVER DISEASE, 2017, 21 (01) :1-+
[8]  
Atia A, 2013, JMRP, V2, P246
[9]   γ-Tocopherol-enriched mixed tocopherol diet inhibits prostate carcinogenesis in TRAMP mice [J].
Barve, Avantika ;
Khor, Tin Oo ;
Nair, Sujit ;
Reuhl, Kenneth ;
Suh, Nanjoo ;
Reddy, Bandaru ;
Newmark, Harold ;
Kong, Ah-Ng .
INTERNATIONAL JOURNAL OF CANCER, 2009, 124 (07) :1693-1699
[10]   Glutathione S-transferase:: differential expression of α, μ, and π isoenzymes in benign prostate, prostatic intraepithelial neoplasia, and prostatic adenocarcinoma [J].
Bostwick, David G. ;
Meiers, Isabelle ;
Shanks, Jonathan H. .
HUMAN PATHOLOGY, 2007, 38 (09) :1394-1401