Gender determination of fertilized unincubated chicken eggs by infrared spectroscopic imaging

被引:43
作者
Steiner, Gerald [1 ]
Bartels, Thomas [2 ]
Stelling, Allison [1 ]
Krautwald-Junghanns, Maria-Elisabeth [2 ]
Fuhrmann, Herbert [3 ]
Sablinskas, Valdas [4 ]
Koch, Edmund [1 ]
机构
[1] Tech Univ Dresden, Fac Med Carl Gustav Carus Clin Sensoring & Monito, D-01307 Dresden, Germany
[2] Univ Leipzig, Fac Vet Med Clin Birds & Reptiles, D-04103 Leipzig, Germany
[3] Univ Leipzig, Inst Physiol Chem, Fac Vet Med, D-04103 Leipzig, Germany
[4] Vilnius State Univ, Dept Gen Phys & Spect, Fac Phys, LT-01108 Vilnius, Lithuania
关键词
Spectroscopy; IR spectroscopy; Bioanalytical methods; EPITHELIAL-CELLS; TISSUE; IDENTIFICATION;
D O I
10.1007/s00216-011-4941-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Each year, billions of day-old layer chicks are produced in the world. Since only female chicks are reared for egg production, the chicks must be sexed and the unwanted male layer chicks are culled. The culling of male chicks is a serious problem, both in terms of animal welfare and waste disposal. The germinal disc in fertilized but unincubated eggs contains already several thousands of blastoderm cells. The cellular DNA in birds is different for male and female chicks. The difference in DNA content between male and female chicks is around 2% and is measurable by Fourier transform infrared (FT-IR) spectroscopy. In this study, small amounts of blastoderm cells from 22 chicken eggs were characterized by attenuated total reflection FT-IR spectroscopic imaging and classified by linear discriminant analysis. Polymerase chain reaction (PCR) was used as a reference method to determine the gender. The spectroscopic results demonstrate that male blastoderm cells exhibit a higher content of DNA than cells from female blastoderm. The spectroscopic-based gender determination led to the same result as the PCR analysis. FT-IR spectroscopic imaging allows the gender determination of unincubated eggs within a few seconds based on the accurate determination of the different DNA contents in blastoderm cells of both sexes.
引用
收藏
页码:2775 / 2782
页数:8
相关论文
共 34 条
[1]  
Aerts S., 2009, ETHICAL FUTURES BIOS, P117
[2]  
[Anonymous], 1980, Infrared Characteristic Group Frequencies
[3]   Primary sex ratios in birds: problems with molecular sex identification of undeveloped eggs [J].
Arnold, KE ;
Orr, KJ ;
Griffiths, R .
MOLECULAR ECOLOGY, 2003, 12 (12) :3451-3458
[4]  
Chiriboga L, 1998, BIOSPECTROSCOPY, V4, P47, DOI 10.1002/(SICI)1520-6343(1998)4:1<47::AID-BSPY5>3.0.CO
[5]  
2-P
[6]   Infrared spectroscopy of human cells and tissue. Part VI: A comparative study of histopathology and infrared microspectroscopy of normal, cirrhotic, and cancerous liver tissue [J].
Chiriboga, L ;
Yee, M ;
Diem, M .
APPLIED SPECTROSCOPY, 2000, 54 (01) :1-8
[7]   Sexing chick embryos: a rapid and simple protocol [J].
Clinton, M ;
Haines, L ;
Belloir, B ;
McBride, D .
BRITISH POULTRY SCIENCE, 2001, 42 (01) :134-138
[8]  
Crawford R.D., 1990, POULTRY BREEDING GEN
[9]   TOUCHDOWN PCR TO CIRCUMVENT SPURIOUS PRIMING DURING GENE AMPLIFICATION [J].
DON, RH ;
COX, PT ;
WAINWRIGHT, BJ ;
BAKER, K ;
MATTICK, JS .
NUCLEIC ACIDS RESEARCH, 1991, 19 (14) :4008-4008
[10]   FTIR spectroscopy studies of nucleic acid damage [J].
Dovbeshko, GI ;
Gridina, NY ;
Kruglova, EB ;
Pashchuk, OP .
TALANTA, 2000, 53 (01) :233-246