Immunoprotection of Japanese Flounder Paralichthys olivaceus by Recombinant Vibrio anguillarum Hemolysin

被引：5
|
作者：
Wang, Hong-Bin [1 ,2 ]
Jiao, Yu-Liang [1 ]
Xu, Jia-Tao [1 ]
Li, Xin-Shu [1 ]
Xu, Bing [1 ]
Dong, Dan [1 ]
Van, Bin-Lun [2 ]
机构：
[1] HuaiHai Inst Technol, Coll Marine Sci, Lianyungang 222005, Peoples R China
[2] Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China
来源：
FISH PATHOLOGY | 2011年 / 46卷 / 01期
关键词：
Vibrio anguillarum; vah4; gene; hemolysin; Paralichthys olivaceus; TROUT;
D O I：
10.3147/jsfp.46.19
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The gene vah4 encoding Vibrio anguillarum hemolysin was cloned and hetelogously expressed. The purified recombinant VAH4 protein was then injected into Japanese flounder Paralichtys olivaceus as an immunogen. Activities of superoxide dismutase, catalase, alkaline phosphatase and acid phosphatase in liver became higher in the immunized group. However, these improvements vanished in 72 h after the injection. VAH4-specific antibodies were detected at a titer of 1:10,240 by ELISA on the 10th day after the injection. In a separate experiment, fish were immunized with VAH4 twice and challenged with V. anguillarum. The immunization with the VAH4 protected fish from V anguillarum infection.
引用
收藏
页码：19 / 22
页数：4
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