Direct assessment of thymic reactivation after autologous stem cell transplantation

被引：30

作者：

Thiel, Andreas ^{[1
,3
]}

Alexander, Tobias ^{[1
,3
]}

Schmidt, Christian A. ^{[8
]}

Przybylski, Gregorsz K. ^{[8
,9
]}

Kimmig, Sonja ^{[6
]}

Kohler, Siegfried ^{[1
]}

Radtke, Hartmut ^{[5
]}

Gromnica-Ihle, Erika ^{[7
]}

Massenkeil, Gero ^{[4
]}

Radbruch, Andreas ^{[2
]}

Arnold, Renate ^{[4
]}

Hiepe, Falk ^{[3
]}

机构：

[1] Deutsches Rheuma Forschungszentrum Berlin, Clin Immunol Grp, DE-10117 Berlin, Germany

[2] Deutsches Rheuma Forschungszentrum Berlin, Cell Biol Grp, DE-10117 Berlin, Germany

[3] Berlin Brandenburg Ctr Regenerat Therapies, Dept Rheumatol, Berlin, Germany

[4] Berlin Brandenburg Ctr Regenerat Therapies, Dept Hematol & Oncol, Berlin, Germany

[5] Univ Hosp Charite, Inst Transfus Med, Berlin, Germany

[6] Max Planck Inst Infect Biol, Dept Mol Biol, Berlin, Germany

[7] Rheuma Klin Berlin Buch, Berlin, Germany

[8] Univ Hosp, Dept Hematol, Greifswald, Germany

[9] Polish Acad Sci, Inst Human Genet, PL-60479 Poznan, Poland

来源：

ACTA HAEMATOLOGICA | 2008年 / 119卷 / 01期

关键词：

thymic activity; T cell; naive; autologous stem cell transplantation; immune reconstitution;

D O I：

10.1159/000117824

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Methods to quantify Th cell reconstitution after immunosuppressive therapies such as hematopoietic stem cell transplantation are becoming a key issue since persistent Th cell deficiencies may result in severe complications and adverse events. We employed here cytometric monitoring of CD31+ thymus-naive Th cells for the direct assessment of human thymic function in 10 patients undergoing autologous stem cell transplantation for severe autoimmune diseases. High frequencies of posttransplant recurring naive Th cells coexpressed CD31 and stable long-term reconstitution with elevated absolute counts of CD31+ thymus-naive Th cells that were enriched with T cell receptor excision circles was demonstrated. Cytometric monitoring of CD31+ thymus-naive Th cells enables to directly evaluate human thymic function ex vivo. Copyright (c) 2008 S. Karger AG, Basel.

引用

页码：22 / 27

页数：6

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