Resveratrol inhibits TGF-β1-induced EMT in gastric cancer cells through Hippo-YAP signaling pathway

被引：24
作者：
Deng, Lei [1 ]
Zou, Jun [2 ]
Su, Yongfeng [1 ]
Wang, Meijian [1 ]
Zhao, Lianwu [3 ]
机构：
[1] Jiangxi Canc Hosp, Affiliated Hosp 2, Jiangxi Clin Res Ctr Canc, Gen Dept Oncol,Nanchang Med Coll, Nanchang 330029, Jiangxi, Peoples R China
[2] Jiangxi Canc Hosp, Affiliated Hosp 2, Jiangxi Clin Res Ctr Canc, Dept Abdominal Tumor Surg,Nanchang Med Coll, Nanchang 330029, Jiangxi, Peoples R China
[3] Nanchang Univ, Affiliated Hosp 1, Dept Gastroenterol, Gaoxin Branch, 7889 Changdong Ave, Nanchang 330029, Jiangxi, Peoples R China
来源：
CLINICAL & TRANSLATIONAL ONCOLOGY | 2022年 / 24卷 / 11期
关键词：
Resveratrol; Hippo-YAP signaling pathway; Epithelial-mesenchymal transition; Proliferation; Migration; Invasion; EPITHELIAL-MESENCHYMAL TRANSITION; INVASION; SUPPRESSES; MIGRATION; METASTASIS; MECHANISMS;
D O I：
10.1007/s12094-022-02882-z
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective To investigate the effects of resveratrol (RSVL) on epithelial-mesenchymal transition (EMT) and biological behaviors of gastric cancer cells. Methods SGC-7901 cells were treated with RSVL, followed by TGF-beta 1 treatment for induction of EMT. Cell proliferation was tested by MTT assay, migration and invasion by Transwell and scratch assays, and Hippo-YAP signaling pathway activation by immunofluorescence. The RNA and protein expressions of E-cadherin, Vimentin, N-cadherin, and Snail were detected by qPCR and Western blot. A tumor model was constructed to examine the effect of RSVL on gastric tumor growth. Results RSVL inhibited the migration, invasion, and growth of gastric cancer cells in concentration- and time-dependent manners. RSVL inhibited TGF-beta 1-induced EMT of gastric cancer cells, which might relate to inactivation of the Hippo-YAP pathway. In the mouse tumor model, RSVL inhibited the EMT process by suppressing the Hippo-YAP pathway. Conclusion RSVL inhibited EMT of gastric cancer cells probably by weakening the Hippo-YAP signaling pathway.
引用
收藏
页码：2210 / 2221
页数：12
相关论文
共 32 条
[1]   Clinical epidemiology of gastric cancer [J].
Ang, Tiing Leong ;
Fock, Kwong Ming .
SINGAPORE MEDICAL JOURNAL, 2014, 55 (12) :621-628
[2]   Olive Leaf Extract Attenuates Inflammatory Activation and DNA Damage in Human Arterial Endothelial Cells [J].
Burja, Blaz ;
Kuret, Tadeja ;
Janko, Tea ;
Topalovic, Dijana ;
Zivkovic, Lada ;
Mrak-Poljsak, Katjusa ;
Spremo-Potparevic, Biljana ;
Zigon, Polona ;
Distler, Oliver ;
Cucnik, Sasa ;
Sodin-Semrl, Snezna ;
Lakota, Katja ;
Frank-Bertoncelj, Mojca .
FRONTIERS IN CARDIOVASCULAR MEDICINE, 2019, 6
[3]   Resveratrol inhibits the hedgehog signaling pathway and epithelial-mesenchymal transition and suppresses gastric cancer invasion and metastasis [J].
Gao, Qian ;
Yuan, Yuan ;
Gan, Hui-Zhong ;
Peng, Qiong .
ONCOLOGY LETTERS, 2015, 9 (05) :2381-2387
[4]   Stearoyl-CoA-desaturase-1 regulates gastric cancer stem-like properties and promotes tumour metastasis via Hippo/YAP pathway [J].
Gao, Yunhe ;
Li, Jiyang ;
Xi, Hongqing ;
Cui, Jianxin ;
Zhang, Kecheng ;
Zhang, Jiabing ;
Zhang, Yanmei ;
Xu, Wei ;
Liang, Wenquan ;
Zhuang, Ziwei ;
Wang, Pengpeng ;
Qiao, Zhi ;
Wei, Bo ;
Chen, Lin .
BRITISH JOURNAL OF CANCER, 2020, 122 (12) :1837-1847
[5]   Anti-tumor effects and cellular mechanisms of resveratrol [J].
Han, Guohua ;
Xia, Jufeng ;
Gao, Jianjun ;
Inagaki, Yoshinori ;
Tang, Wei ;
Kokudo, Norihiro .
DRUG DISCOVERIES AND THERAPEUTICS, 2015, 9 (01) :1-12
[6]   Sauchinone prevents TGF-β-induced EMT and metastasis in gastric cancer cells [J].
He, Zhikuan ;
Dong, Wenxing ;
Li, Quanying ;
Qin, Changjiang ;
Li, Yongjun .
BIOMEDICINE & PHARMACOTHERAPY, 2018, 101 :355-361
[7]   Combined Ischemic Preconditioning and Resveratrol Improved Blood-brain Barrier Breakdown via Hippo/YAP/TAZ Signaling Pathway [J].
Hong, Ganji ;
Yan, Ying ;
Zhong, Yali ;
Chen, Jianer ;
Tong, Fei ;
Ma, Qilin .
CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS, 2019, 18 (09) :713-722
[8]   Anti-metastatic potential of resveratrol and its metabolites by the inhibition of epithelial-mesenchymal transition, migration, and invasion of malignant cancer cells [J].
Kim, Cho-Won ;
Hwang, Kyung-A ;
Choi, Kyung-Chul .
PHYTOMEDICINE, 2016, 23 (14) :1787-1796
[9]   Resveratrol suppresses gastric cancer cell proliferation and survival through inhibition of PIM-1 kinase activity [J].
Kim, Sujin ;
Kim, Wonki ;
Kim, Do-Hee ;
Jang, Jeong-Hoon ;
Kim, Su-Jung ;
Park, Sin-Aye ;
Hahn, Hyunggu ;
Han, Byung Woo ;
Na, Hye-Kyung ;
Chun, Kyung-Soo ;
Choi, Bu Young ;
Surh, Young-Joon .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2020, 689
[10]   Resveratrol suppresses breast cancer cell invasion by inactivating a RhoA/YAP signaling axis [J].
Kim, Yu Na ;
Choe, So Ra ;
Cho, Kyung Hwa ;
Cho, Do Yeun ;
Kang, Jaeku ;
Park, Chang Gyo ;
Lee, Hoi Young .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2017, 49 :e296-e296
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