Resveratrol inhibits TGF-β1-induced EMT in gastric cancer cells through Hippo-YAP signaling pathway

被引：17
|
作者：
Deng, Lei [1 ]
Zou, Jun [2 ]
Su, Yongfeng [1 ]
Wang, Meijian [1 ]
Zhao, Lianwu [3 ]
机构：
[1] Jiangxi Canc Hosp, Affiliated Hosp 2, Jiangxi Clin Res Ctr Canc, Gen Dept Oncol,Nanchang Med Coll, Nanchang 330029, Jiangxi, Peoples R China
[2] Jiangxi Canc Hosp, Affiliated Hosp 2, Jiangxi Clin Res Ctr Canc, Dept Abdominal Tumor Surg,Nanchang Med Coll, Nanchang 330029, Jiangxi, Peoples R China
[3] Nanchang Univ, Affiliated Hosp 1, Dept Gastroenterol, Gaoxin Branch, 7889 Changdong Ave, Nanchang 330029, Jiangxi, Peoples R China
来源：
CLINICAL & TRANSLATIONAL ONCOLOGY | 2022年 / 24卷 / 11期
关键词：
Resveratrol; Hippo-YAP signaling pathway; Epithelial-mesenchymal transition; Proliferation; Migration; Invasion; EPITHELIAL-MESENCHYMAL TRANSITION; INVASION; SUPPRESSES; MIGRATION; METASTASIS; MECHANISMS;
D O I：
10.1007/s12094-022-02882-z
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective To investigate the effects of resveratrol (RSVL) on epithelial-mesenchymal transition (EMT) and biological behaviors of gastric cancer cells. Methods SGC-7901 cells were treated with RSVL, followed by TGF-beta 1 treatment for induction of EMT. Cell proliferation was tested by MTT assay, migration and invasion by Transwell and scratch assays, and Hippo-YAP signaling pathway activation by immunofluorescence. The RNA and protein expressions of E-cadherin, Vimentin, N-cadherin, and Snail were detected by qPCR and Western blot. A tumor model was constructed to examine the effect of RSVL on gastric tumor growth. Results RSVL inhibited the migration, invasion, and growth of gastric cancer cells in concentration- and time-dependent manners. RSVL inhibited TGF-beta 1-induced EMT of gastric cancer cells, which might relate to inactivation of the Hippo-YAP pathway. In the mouse tumor model, RSVL inhibited the EMT process by suppressing the Hippo-YAP pathway. Conclusion RSVL inhibited EMT of gastric cancer cells probably by weakening the Hippo-YAP signaling pathway.
引用
收藏
页码：2210 / 2221
页数：12
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