Identification of volatile biomarkers for high-throughput sensing of soft rot and Pythium leak diseases in stored potatoes

被引：14

作者：

Sangjan, Worasit ^{[1
]}

Marzougui, Afef ^{[1
]}

Mattinson, D. Scott ^{[2
]}

Schroeder, Brenda K. ^{[3
]}

Bates, Austin A. ^{[3
]}

Khot, Lav R. ^{[1
,4
]}

Sankaran, Sindhuja ^{[1
,4
]}

机构：

[1] Washington State Univ, Dept Biol Syst Engn, 1935 E Grimes Way,POB 646120, Pullman, WA 99164 USA

[2] Washington State Univ, Dept Hort, POB 646414, Pullman, WA 99164 USA

[3] Univ Idaho, Dept Entomol Plant Pathol & Nematol, 875 Perimeter Dr MS 2329, Moscow, ID 83843 USA

[4] Washington State Univ, Ctr Precis & Automated Agr Syst, 24106 N Bunn Rd, Prosser, WA USA

来源：

FOOD CHEMISTRY | 2022年 / 370卷

基金：

美国食品与农业研究所;

关键词：

Potato rot; Disease detection; Volatile organic compounds; Headspace sampling; Solid-phase microextraction; Gas chromatography; ERWINIA-CAROTOVORA; ORGANIC-COMPOUNDS; GC; PECTOBACTERIUM; INFECTION;

D O I：

10.1016/j.foodchem.2021.130910

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Soft rot and Pythium leak are postharvest storage diseases of potato tubers that can cause substantial crop losses in the US. This study focused on detecting volatile organic compounds (VOCs) associated with rot inoculated tubers during storage (up to 21 days) using headspace solid-phase microextraction (SPME) coupled to gas chromatography (GC) with mass spectrometry (MS) and flame ionization detector (FID) analysis. Russet Burbank and Ranger Russet tubers were inoculated with the rot pathogens. Static sampling with 50 min trapping time followed by GC-MS and GC-FID analysis identified 23 and 30 common VOCs from the pathogen inoculated tubers. Overall, n,n-dimethylmethylamine, acetone, 1-undecene, and styrene, occurred frequently and repeatability in inoculated samples based on GC-MS analysis, with the latter two found using GC-FID analysis as well. Identification of such biomarkers can be useful in developing high-throughput VOC sensing systems for early disease detection in potato storage facilities.

引用

页数：9

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