In vivo tumor growth of high-grade serous ovarian cancer cell lines

被引：160

作者：

Mitra, Anirban K. ^{[1
]}

Davis, David A. ^{[2
]}

Tomar, Sunil ^{[1
]}

Roy, Lynn ^{[3
,4
]}

Gurler, Hilal ^{[6
]}

Xie, Jia ^{[6
]}

Lantvit, Daniel D. ^{[2
]}

Cardenas, Horacio ^{[7
]}

Fang, Fang ^{[1
]}

Liu, Yueying ^{[4
,5
]}

Loughran, Elizabeth ^{[4
,5
]}

Yang, Jing ^{[4
,5
]}

Stack, M. Sharon ^{[4
,5
]}

Emerson, Robert E. ^{[8
]}

Dahl, Karen D. Cowden ^{[3
,4
,5
]}

Barbolina, Maria V. ^{[6
]}

Nephew, Kenneth P. ^{[1
,9
]}

Matei, Daniela ^{[7
]}

Burdette, Joanna E. ^{[2
]}

机构：

[1] Indiana Univ, Indiana Univ Sch Med, Med Sci Program, Bloomington, IN 47405 USA

[2] Univ Illinois, Dept Med Chem & Pharmacognosy, Chicago, IL USA

[3] Indiana Univ Sch Med South Bend, Dept Biochem & Mol Biol, South Bend, IN USA

[4] Harper Canc Res Inst, Notre Dame, IN USA

[5] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA

[6] Univ Illinois, Dept Biopharmaceut Sci, Chicago, IL USA

[7] Indiana Univ Sch Med, Dept Med, Indianapolis, IN 46202 USA

[8] Indiana Univ Sch Med, Dept Pathol, Indianapolis, IN 46202 USA

[9] Indiana Univ Sch Med, Dept Cellular & Integrat Physiol, Indianapolis, IN 46202 USA

来源：

GYNECOLOGIC ONCOLOGY | 2015年 / 138卷 / 02期

关键词：

High grade serous ovarian cancer; Xenograft; Pax8; Mouse model; TUBE EPITHELIAL-CELLS; PERSPECTIVES;

D O I：

10.1016/j.ygyno.2015.05.040

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objective. Genomic studies of ovarian cancer (OC) cell lines frequently used in research revealed that these cells do not fully represent high-grade serous ovarian cancer (HGSOC), the most common OC histologic type. However, OC lines that appear to genomically resemble HGSOC have not been extensively used and their growth characteristics in murine xenografts are essentially unknown. Methods. To better understand growth patterns and characteristics of HGSOC cell lines in vivo, CAOV3, COV362, KURAMOCHI, NIH-OVCAR3, OVCAR4, OVCAR5, OVCAR8, OVSAHO, OVKATE, SNU119 and UWB1.289 cells were assessed for tumor formation in nude mice. Cells were injected intraperitoneally (i.p.) or subcutaneously (s.c.) in female athymic nude mice and allowed to grow (maximum of 90 days) and tumor formation was analyzed. All tumors were sectioned and assessed using H&E staining and immunohistochemistry for p53, PAX8 and WT1 expression. Results. Six lines (OVCAR3, OVCAR4, OVCAR5, OVCAR8, CAOV3, and OVSAHO) formed i.p xenografts with HGSOC histology. OVKATE and COV362 formed s.c. tumors only. Rapid tumor formation was observed for OVCAR3, OVCAR5 and OVCAR8, but only OVCAR8 reliably formed ascites. Tumors derived from OVCAR3, OVCAR4, and OVKATE displayed papillary features. Of the 11 lines examined, three (Kuramochi, SNU119 and UWB1.289) were non-tumorigenic. Conclusions. Our findings help further define which HGSOC cell models reliably generate tumors and/or ascites, critical information for preclinical drug development, validating in vitro findings, imaging and prevention studies by the OC research community. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：372 / 377

页数：6

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