Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay

被引：44
作者：
Gilbert, SA
Larochelle, R
Magar, R
Cho, HJ
Deregt, D
机构：
[1] AGR CANADA,ANIM DIS RES INST,LETHBRIDGE,AB T1J 3Z4,CANADA
[2] AGR & AGROALIMENTAIRE,LAB HYG VET & ALIMENTAIRE,ST HYACINTHE,PQ,CANADA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 01期
关键词：
D O I：
10.1128/JCM.35.1.264-267.1997
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A rapid multiplex PCR assay was developed to distinguish between North American and European genotypes of porcine reproductive and respiratory syndrome (PRRS) virus after a portion of the polymerase gene (open reading frame 1b) was sequenced for two North American PRRS virus strains. DNA products with unique sizes characteristic of each genotype were obtained.
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页码：264 / 267
页数：4
相关论文
共 29 条
[1]   SEROLOGIC SURVEY FOR LELYSTAD AND VR-2332 STRAINS OF PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME (PRRS) VIRUS IN US SWINE HERDS [J].
BAUTISTA, EM ;
GOYAL, SM ;
COLLINS, JE .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (04) :612-614
[2]   DETECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN BOAR SEMEN BY PCR [J].
CHRISTOPHERHENNINGS, J ;
NELSON, EA ;
NELSON, JK ;
HINES, RJ ;
SWENSON, SL ;
HILL, HT ;
ZIMMERMAN, JJ ;
KATZ, JB ;
YAEGER, MJ ;
CHASE, CCL ;
BENFIELD, DA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (07) :1730-1734
[3]   ISOLATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME VIRUS (ISOLATE ATCC VR-2332) IN NORTH-AMERICA AND EXPERIMENTAL REPRODUCTION OF THE DISEASE IN GNOTOBIOTIC PIGS [J].
COLLINS, JE ;
BENFIELD, DA ;
CHRISTIANSON, WT ;
HARRIS, L ;
HENNINGS, JC ;
SHAW, DP ;
GOYAL, SM ;
MCCULLOUGH, S ;
MORRISON, RB ;
JOO, HS ;
GORCYCA, D ;
CHLADEK, D .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) :117-126
[4]   MOLECULAR CHARACTERIZATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS, A MEMBER OF THE ARTERIVIRUS GROUP [J].
CONZELMANN, KK ;
VISSER, N ;
VANWOENSEL, P ;
THIEL, HJ .
VIROLOGY, 1993, 193 (01) :329-339
[5]   PRODUCTION, CHARACTERIZATION AND REACTIVITY OF MONOCLONAL-ANTIBODIES TO PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS [J].
DREW, TW ;
MEULENBERG, JJM ;
SANDS, JJ ;
PATON, DJ .
JOURNAL OF GENERAL VIROLOGY, 1995, 76 :1361-1369
[6]  
GILBERT SA, 1995, FOCUS, V17, P18
[7]   PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME [J].
GOYAL, SM .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (04) :656-664
[8]   ANTIGENIC DIFFERENCES BETWEEN EUROPEAN AND AMERICAN ISOLATES OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) ARE ENCODED BY THE CARBOXYTERMINAL PORTION OF VIRAL OPEN READING FRAME-3 [J].
KATZ, JB ;
SHAFER, AL ;
EERNISSE, KA ;
LANDGRAF, JG ;
NELSON, EA .
VETERINARY MICROBIOLOGY, 1995, 44 (01) :65-76
[9]  
KEFFABER KK, 1989, AM ASS SWINE PRACT N, V2, P1
[10]   ENHANCED REPLICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS IN A HOMOGENEOUS SUBPOPULATION OF MA-104 CELL-LINE [J].
KIM, HS ;
KWANG, J ;
YOON, IJ ;
JOO, HS ;
FREY, ML .
ARCHIVES OF VIROLOGY, 1993, 133 (3-4) :477-483
123