Over-expression of wild-type Securin leads to aneuploidy in human cells

被引：17
作者：
Christopoulou, L
Moore, JD
Tyler-Smith, C
机构：
[1] Univ Oxford, Dept Biochem, CRC Chromosome Mol Biol Grp, Oxford OX1 3QU, England
[2] Canc Res UK, London Res Inst, Clare Hall Labs, Cell Cycle Control Lab, S Mimms EN6 3LD, Herts, England
来源：
CANCER LETTERS | 2003年 / 202卷 / 02期
基金：
英国生物技术与生命科学研究理事会;
关键词：
aneuploidy; human cells; chromosome loss assay; protein over-expression; Securin;
D O I：
10.1016/S0304-3835(03)00483-X
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Changes in gene expression and aneuploidy are both common features of cancers and under- or over-expression of single genes can cause aneuploidy. Securin, which is involved in chromatid separation, has transforming activity in vitro and is over-expressed in many tumours. We have used a GFP-based assay for chromosome loss in human cells to investigate the effects of over-expressing Securin. We find that over-expression causes a two-fold increase in chromosome loss rate and can thus be a direct cause of aneuploidy. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：213 / 218
页数：6
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