Pseudo 3D single-walled carbon nanotube film for BSA-free protein chips

被引：12
作者：
Byon, HR
Hong, BJ
Gho, YS
Park, JW
Choi, HC
机构：
[1] Pohang Univ Sci & Technol, Dept Chem, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea
来源：
CHEMBIOCHEM | 2005年 / 6卷 / 08期
关键词：
BSA-free system; microarrays; protein chips; nanotubes;
D O I：
10.1002/cbic.200500081
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sweet home for proteins. Single-walled carbon nanotube (SWNT) film treated with CDI-Tween20 (CT) has been used as a substrate for a BSA-free protein chip. In the absence of BSA, which is conventionally applied as a nonspecific binding inhibitor, protein A, biotinylated BSA, and even small peptide such as 3 × FLAG immobilized on the CT-functionalized SWNT film substrates recognize their respective counterparts, streptavidin, immunoglobulin G, and antiFLAG. with high specificity. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1331 / 1334
页数：4
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