In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing

被引：92
作者：
Bi, Hongyan [1 ]
Feng, Tianyi [1 ]
Li, Binbin [1 ]
Han, Yingchao [1 ]
机构：
[1] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Biomed Mat & Engn Res Ctr Hubei Prov, Wuhan 430070, Peoples R China
来源：
POLYMERS | 2020年 / 12卷 / 04期
关键词：
tissue repair; wound healing; wound dressing; fiber membrane; electrospinning; POLYLACTIC ACID; DRESSINGS; SCAFFOLDS; NANOFIBERS;
D O I：
10.3390/polym12040839
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Wound dressings can accelerate wound healing. The degradable polymer poly(lactic acid) (PLA) shows good mechanical properties and biocompatibility. Sodium alginate (SA) holds good biocompatibility, hemostasis, and high hygroscopicity. Poly(vinyl alcohol) (PVA) has good spinnability as a pharmaceutical excipient. Herein, we carried out a comparison study of electrospun PLA and PLA/PVA/SA fiber membranes for wound healing in vitro and in vivo. In this study, PLA and PLA/PVA/SA nanofiber membranes were fabricated through electrospinning to produce a highly porous and large specific surface area that could promote wound healing. In vitro experiments showed that PLA and PLA/PVA/SA nanofiber membranes could all provide good support for the growth of rat fibroblasts (L929). Moreover, rat fibroblasts displayed slightly better adhesion and proliferation on PLA/PVA/SA than on the PLA fiber membranes. The in vivo potentiality of the PLA and PLA/PVA/SA fiber membranes was assessed in rat models of skin defects in which the PLA and PLA/PVA/SA fiber membranes significantly improved wound healing compared to commercially available gauzes. No significant differences in wound healing were observed between PLA and PLA/PVA/SA fiber membranes in our study. Furthermore, Masson staining and PCR displayed the PLA fiber membrane promoted protein deposition compared to the PLA/PVA/SA fiber membrane. In addition, IHC suggested that PLA/PVA/SA dressing reduced the inflammatory response during early wound healing compared to the PLA fiber membrane. These findings highlight the utility of fiber membranes as novel wound-healing dressings.
引用
收藏
页数：13
相关论文
共 38 条
[11]   Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications [J].
Garcia-Orue, Itxaso ;
Gainza, Garazi ;
Borja Gutierrez, Franciso ;
Javier Aguirre, Jose ;
Evora, Carmen ;
Luis Pedraz, Jose ;
Maria Hernandez, Rosa ;
Delgado, Araceli ;
Igartua, Manoli .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2017, 523 (02) :556-566
[12]   Wound repair and regeneration [J].
Gurtner, Geoffrey C. ;
Werner, Sabine ;
Barrandon, Yann ;
Longaker, Michael T. .
NATURE, 2008, 453 (7193) :314-321
[13]   Properties and medical applications of polylactic acid: A review [J].
Hamad, K. ;
Kaseem, M. ;
Yang, H. W. ;
Deri, F. ;
Ko, Y. G. .
EXPRESS POLYMER LETTERS, 2015, 9 (05) :435-455
[14]   Engineered electrospun polycaprolactone (PCL)/octacalcium phosphate (OCP) scaffold for bone tissue engineering [J].
Heydari, Zohre ;
Mohebbi-Kalhori, Davod ;
Afarani, Mandi Shafiee .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2017, 81 :127-132
[15]   Effect of chitosan-polyvinyl alcohol blend nanofibrous web on the healing of excision and incision full thickness wounds [J].
Kanani, A. G. ;
Bahrami, S. H. ;
Taftei, H. A. ;
Rabbani, S. ;
Sotoudeh, M. .
IET NANOBIOTECHNOLOGY, 2010, 4 (04) :109-117
[16]   Poly(N-isopropylacrylamide)/poly(L-lactic acid-co-ε-caprolactone) fibers loaded with ciprofloxacin as wound dressing materials [J].
Li, Heyu ;
Williams, Gareth R. ;
Wu, Junzi ;
Wang, Haijun ;
Sun, Xiaozhu ;
Zhu, Li-Min .
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2017, 79 :245-254
[17]   A conducive bioceramic/polymer composite biomaterial for diabetic wound healing [J].
Lv, Fang ;
Wang, Jie ;
Xu, Peng ;
Han, Yiming ;
Ma, Hongshi ;
Xu, He ;
Chen, Shijie ;
Chang, Jiang ;
Ke, Qinfei ;
Liu, Mingyao ;
Yi, Zhengfang ;
Wu, Chengtie .
ACTA BIOMATERIALIA, 2017, 60 :128-143
[18]   Electrospun nanostructured scaffolds for tissue engineering applications [J].
Martins, Albino ;
Araujo, Jose V. ;
Reis, Rui L. ;
Neves, Nuno M. .
NANOMEDICINE, 2007, 2 (06) :929-942
[19]   Preparation of Chitosan, Sodium Alginate, Gelatin and Collagen Biodegradable Sponge Composites and their Application in Wound Healing and Curcumin Delivery [J].
Naghshineh, Negar ;
Tahvildari, Kambiz ;
Nozari, Maryam .
JOURNAL OF POLYMERS AND THE ENVIRONMENT, 2019, 27 (12) :2819-2830
[20]   Electrospun preparation and biological properties in vitro of polyvinyl alcohol/sodium alginate/nano-hydroxyapatite composite fiber membrane [J].
Ni, Peilong ;
Bi, Hongyan ;
Zhao, Gang ;
Han, Yingchao ;
Wickramaratne, M. Nirmali ;
Dai, Honglian ;
Wang, Xinyu .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2019, 173 :171-177
1234