In Vitro and In Vivo Comparison Study of Electrospun PLA and PLA/PVA/SA Fiber Membranes for Wound Healing

被引：89

作者：

Bi, Hongyan ^{[1
]}

Feng, Tianyi ^{[1
]}

Li, Binbin ^{[1
]}

Han, Yingchao ^{[1
]}

机构：

[1] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Biomed Mat & Engn Res Ctr Hubei Prov, Wuhan 430070, Peoples R China

来源：

POLYMERS | 2020年 / 12卷 / 04期

关键词：

tissue repair; wound healing; wound dressing; fiber membrane; electrospinning; POLYLACTIC ACID; DRESSINGS; SCAFFOLDS; NANOFIBERS;

D O I：

10.3390/polym12040839

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Wound dressings can accelerate wound healing. The degradable polymer poly(lactic acid) (PLA) shows good mechanical properties and biocompatibility. Sodium alginate (SA) holds good biocompatibility, hemostasis, and high hygroscopicity. Poly(vinyl alcohol) (PVA) has good spinnability as a pharmaceutical excipient. Herein, we carried out a comparison study of electrospun PLA and PLA/PVA/SA fiber membranes for wound healing in vitro and in vivo. In this study, PLA and PLA/PVA/SA nanofiber membranes were fabricated through electrospinning to produce a highly porous and large specific surface area that could promote wound healing. In vitro experiments showed that PLA and PLA/PVA/SA nanofiber membranes could all provide good support for the growth of rat fibroblasts (L929). Moreover, rat fibroblasts displayed slightly better adhesion and proliferation on PLA/PVA/SA than on the PLA fiber membranes. The in vivo potentiality of the PLA and PLA/PVA/SA fiber membranes was assessed in rat models of skin defects in which the PLA and PLA/PVA/SA fiber membranes significantly improved wound healing compared to commercially available gauzes. No significant differences in wound healing were observed between PLA and PLA/PVA/SA fiber membranes in our study. Furthermore, Masson staining and PCR displayed the PLA fiber membrane promoted protein deposition compared to the PLA/PVA/SA fiber membrane. In addition, IHC suggested that PLA/PVA/SA dressing reduced the inflammatory response during early wound healing compared to the PLA fiber membrane. These findings highlight the utility of fiber membranes as novel wound-healing dressings.

引用

页数：13

共 38 条

[1] Electrospun Nanofibers as Dressings for Chronic Wound Care: Advances, Challenges, and Future Prospects [J].

Abrigo, Martina ;

McArthur, Sally L. ;

Kingshott, Peter .

MACROMOLECULAR BIOSCIENCE, 2014, 14 (06) :772-792

[2] Electrospun chitosan-based nanofibers and their cellular compatibility [J].

Bhattarai, N ;

Edmondson, D ;

Veiseh, O ;

Matsen, FA ;

Zhang, MQ .

BIOMATERIALS, 2005, 26 (31) :6176-6184

[3] Novel biodegradable electrospun membrane: scaffold for tissue engineering [J].

Bhattarai, SR ;

Bhattarai, N ;

Yi, HK ;

Hwang, PH ;

Cha, DI ;

Kim, HY .

BIOMATERIALS, 2004, 25 (13) :2595-2602

[4]

Brown A. L., 2001, ACS SYM SER, V792, P90

[5] In situ reduction of silver nanoparticles by sodium alginate to obtain silver-loaded composite wound dressing with enhanced mechanical and antimicrobial property [J].

Chen, Kai ;

Wang, Fengyan ;

Liu, Siyu ;

Wu, Xiaofang ;

Xu, Linmin ;

Zhang, Dekun .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 148 :501-509

[6] Facile preparation of polysaccharide-based sponges and their potential application in wound dressing [J].

Cheng, Huan ;

Li, Chaojing ;

Jiang, Yujia ;

Wang, Bijia ;

Wang, Fujun ;

Mao, Zhiping ;

Xu, Hong ;

Wang, Lu ;

Sui, Xiaofeng .

JOURNAL OF MATERIALS CHEMISTRY B, 2018, 6 (04) :634-640

[7] Electrospinning versus microfluidic spinning of functional fibers for biomedical applications [J].

Cheng, Jie ;

Jun, Yesl ;

Qin, Jianhua ;

Lee, Sang-Hoon .

BIOMATERIALS, 2017, 114 :121-143

[8] Advances in electrospun skin substitutes [J].

Dias, J. R. ;

Granja, P. L. ;

Bartolo, P. J. .

PROGRESS IN MATERIALS SCIENCE, 2016, 84 :314-334

[9]

Drumright RE, 2000, ADV MATER, V12, P1841, DOI 10.1002/1521-4095(200012)12:23<1841::AID-ADMA1841>3.0.CO

[10]

2-E

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