LC-MS/MS-analysis of prostaglandin E2 and D2 in microdialysis samples of rats

被引：56
作者：
Schmidt, R [1 ]
Coste, O [1 ]
Geisslinger, G [1 ]
机构：
[1] Goethe Univ Frankfurt, Klilikum Univ Frankfurt, Inst Klin Pharmakol, Pharmazentrum Frankfurt,ZAFES, D-60590 Frankfurt, Germany
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 826卷 / 1-2期
关键词：
PGE(2); PGD(2); chromatography; tandem mass spectrometry; ESI; microdialysis;
D O I：
10.1016/j.jchromb.2005.09.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
For the determination of prostaglandins in microdialysis samples, usually immunoassays are used. However, these assays may show cross-reactivity among various prostaglandins. To overcome this problem a specific method for the determination of prostaglandin E-2 and D-2 in rat microdialysis samples by using liquid chromatography-electrospay ionization-tandem mass spectrometry (LC-ESI-MS/MS) is described. Prostaglandin E-2 and D-2 were extracted from microdialysis samples with liquid-liquid extraction using deuterated prostaglandin D-2, [H-2(4)]-PGD(2), as internal standard. Subsequently, prostaglandins were separated with a phenomenex Synergi Hydro-RP column and determined with a PE Sciex API 3000 mass spectrometer equipped with a turbo ion spray interface operating in negative ionization mode. The method showed a LLOQ of 25 pg/ml for prostaglandin E, and 50 pg/ml for prostaglandin D-2. The applicability of the method is shown in rat spinal cord microdialysis samples following peripheral nociceptive stimulation. (c) 2005 Elsevier B.V All rights reserved.
引用
收藏
页码：188 / 197
页数：10
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