Construction of an electronic physical map of Magnaporthe oryzae using genomic position-ready SSR markers

被引：10
作者：
Feng ShuJie [1 ,2 ]
Ma JunHong [1 ]
Lin Fei [1 ]
Wang Ling [1 ]
Pan QingHua [1 ]
机构：
[1] S China Agr Univ, Coll Nat Resources & Environm Sci, Lab Plant Resistance & Genet, Guangzhou 510642, Guangdong, Peoples R China
[2] S China Agr Univ, Coll Horticulture, Lab Plant Protect, Guangzhou 510642, Guangdong, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2007年 / 52卷 / 24期
关键词：
Magnaporthe oryzae; simple sequence repeat (SSR); reference sequence; physical map; in silico mapping;
D O I：
10.1007/s11434-007-0498-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat (SSR)-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were locatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr (avirulence) genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolutionally related pathogens.
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页码：3346 / 3354
页数：9
相关论文
共 54 条
[1]   Microsatellite DNA markers for rice chromosomes [J].
Akagi, H ;
Yokozeki, Y ;
Inagaki, A ;
Fujimura, T .
THEORETICAL AND APPLIED GENETICS, 1996, 93 (07) :1071-1077
[2]   High genetic diversity, phenotypic uniformity, and evidence of outcrossing in Sclerotinia sclerotiorum in the Columbia basin of Washington state [J].
Atallah, ZK ;
Larget, B ;
Chen, X ;
Johnson, DA .
PHYTOPATHOLOGY, 2004, 94 (07) :737-742
[3]   A putative polyketide synthase peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice [J].
Böhnert, HU ;
Fudal, I ;
Dioh, W ;
Tharreau, D ;
Notteghem, JL ;
Lebrun, MH .
PLANT CELL, 2004, 16 (09) :2499-2513
[4]   Development of microsatellite markers for the genetic analysis of Magnaporthe grisea [J].
Brondani, C ;
Brondani, RPV ;
Garrido, LD ;
Ferreira, ME .
GENETICS AND MOLECULAR BIOLOGY, 2000, 23 (04) :753-762
[5]  
BUDDE AD, 1993, GENETICS MAPS, P3110
[6]   Microsatellite polymorphism in Pisum sativum [J].
Burstin, J ;
Deniot, G ;
Potier, J ;
Weinachter, C ;
Aubert, G ;
Baranger, A .
PLANT BREEDING, 2001, 120 (04) :311-317
[7]   Genetic analysis and molecular mapping of the avirulence gene PRE1, a gene for host-species specificity in the blast fungus Magnaporthe grisea [J].
Chen, Q. H. ;
Wang, Y. C. ;
Zheng, X. B. .
GENOME, 2006, 49 (08) :873-881
[8]   Sequence divergence of rice microsatellites in Oryza and other plant species [J].
Chen, X ;
Cho, YG ;
McCouch, SR .
MOLECULAR GENETICS AND GENOMICS, 2002, 268 (03) :331-343
[9]   Diversity of microsatellites derived from genomic libraries and GenBank sequences in rice (Oryza sativa L.) [J].
Cho, YG ;
Ishii, T ;
Temnykh, S ;
Chen, X ;
Lipovich, L ;
McCouch, SR ;
Park, WD ;
Ayres, N ;
Cartinhour, S .
THEORETICAL AND APPLIED GENETICS, 2000, 100 (05) :713-722
[10]   The genome sequence of the rice blast fungus Magnaporthe grisea [J].
Dean, RA ;
Talbot, NJ ;
Ebbole, DJ ;
Farman, ML ;
Mitchell, TK ;
Orbach, MJ ;
Thon, M ;
Kulkarni, R ;
Xu, JR ;
Pan, HQ ;
Read, ND ;
Lee, YH ;
Carbone, I ;
Brown, D ;
Oh, YY ;
Donofrio, N ;
Jeong, JS ;
Soanes, DM ;
Djonovic, S ;
Kolomiets, E ;
Rehmeyer, C ;
Li, WX ;
Harding, M ;
Kim, S ;
Lebrun, MH ;
Bohnert, H ;
Coughlan, S ;
Butler, J ;
Calvo, S ;
Ma, LJ ;
Nicol, R ;
Purcell, S ;
Nusbaum, C ;
Galagan, JE ;
Birren, BW .
NATURE, 2005, 434 (7036) :980-986
123456