Single-stranded DNAzyme-based Pb2+ fluorescent sensor that can work well over a wide temperature range

被引:45
作者
Li, Hui [1 ]
Zhang, Qi [1 ]
Cai, Yang [1 ]
Kong, De-Ming [1 ]
Shen, Han-Xi [1 ]
机构
[1] Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
关键词
DNAzyme; Metal biosensor; Fluorescent sensor; Pb2+; Single-stranded DNAzyme; IN-VITRO SELECTION; MOLECULAR BEACONS; DEOXYRIBOZYMES; LEAD; SENSITIVITY; ABSORPTION; IONS;
D O I
10.1016/j.bios.2012.01.037
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
DNAzymes have become an excellent choice for sensing applications. Based on DNAzymes, three generations of Pb2+ fluorescent sensors have been reported. In these sensors, two oligonucleotide strands (substrate strand and enzyme strand) were used, which not only increased the complexity of the detection system, but also brought some difficulties for the use of the sensors at elevated temperatures. To overcome this problem, a single-stranded DNAzyme-based Pb2+ fluorescent sensor was designed by combining the substrate sequence and the enzyme sequence into one oligonucleotide strand. The intramolecular duplex structure of this single-stranded DNAzyme kept the fluorophore and the quencher, labeled at its two ends, in close proximity; thus the background fluorescence was significantly suppressed. Using this fluorescent sensor, Pb2+ quantitation can be achieved with high sensitivity and high selectivity. In addition, the extraordinary stability of the intramolecular duplex structure could assure a low background fluorescence at high temperature, even if the number of complementary base pairs between the substrate sequence and the enzyme sequence was reduced, allowing the sensor to work well over a wide temperature range. Similar performances of the fluorescent sensor at 4, 25 and 37 degrees C suggested that this sensor has a good ability to resist temperature fluctuations. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:159 / 164
页数:6
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