Development of ten microsatellite markers for Quercus mongolica var. crispula by database mining

被引：27

作者：

Ueno, Saneyoshi ^{[1
]}

Tsumura, Yoshihiko ^{[1
]}

机构：

[1] Forestry & Forest Prod Res Inst, Dept Forest Genet, Tree Genet Lab, Tsukuba, Ibaraki 3058687, Japan

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 04期

关键词：

expressed sequence tag; fagaceae; Quercus petraea; Quercus robur; SSR;

D O I：

10.1007/s10592-007-9462-4

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Simple sequence repeats (SSRs) in the NCBI dbEST database were surveyed to identify potential SSR markers for Quercus mongolica. In total, 2,691 gene sequences, mainly from expressed sequence tags (ESTs) for Q. robur and Q. petraea had been registered. Twenty-two PCR primers were designed for SSRs in these sequences and screened for polymorphisms in 16 Q. mongolica trees. Ten loci were easily genotyped and showed polymorphism, with numbers of alleles and expected heterozygosity ranging from 3 to 15 and 0.28 to 0.94, respectively. These EST-SSR markers should be useful for studying the genetic diversity of Quercus species.

引用

页码：1083 / 1085

页数：3

共 8 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].