Protein kinase D regulates the human cardiac L-type voltage-gated calcium channel through serine 1884

被引：9

作者：

Aita, Yusuke ^{[2
]}

Kurebayashi, Nagomi ^{[3
]}

Hirose, Shigehisa ^{[2
]}

Maturana, Andres D. ^{[1
,4
]}

机构：

[1] Tokyo Inst Technol, Global Edge Inst, Tokyo 1528550, Japan

[2] Tokyo Inst Technol, Dept Biol Sci, Yokohama, Kanagawa 2268501, Japan

[3] Juntendo Univ, Grad Sch Med, Dept Cellular & Mol Pharmacol, Tokyo 1138421, Japan

[4] Nagaoka Univ Technol, Dept Bioengn, Niigata 9402188, Japan

来源：

FEBS LETTERS | 2011年 / 585卷 / 24期

关键词：

Human voltage-gated calcium channel; Protein kinase D; CA(V)1.2 PHOSPHORYLATION; SITE;

D O I：

10.1016/j.febslet.2011.11.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein kinase D (PKD) regulates the activity of the L-type calcium channel in rat ventricular cardiomyocytes. However, the functional target residues of PKD on the L-type calcium channel remain to be identified. Our aim was to identify the functional phosphorylation sites of PKD on the human L-type calcium channel. The pore subunit of the human CaV1.2 (hCaV1.2) was stably expressed in HEK293 cells. Both the expression of a dominant-negative mutant of PKD and the mutation of serine 1884 but not serine 1930, putative targets of PKD, strongly reduced L-type calcium currents and single channel activity without affecting the channel's expression at the plasma membrane. Our results suggest that serine 1884 is essential for the regulation of hCaV1.2 by PKD. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3903 / 3906

页数：4

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