Detection of invasive infection caused by Fusarium solani and non-Fusarium solani species using a duplex quantitative PCR-based assay in a murine model of fusariosis

被引：25
作者：
Bernal-Martinez, Leticia [1 ]
Buitrago, Maria J. [1 ]
Castelli, Maria V. [1 ]
Rodriguez-Tudela, Juan L. [1 ]
Cuenca-Estrella, Manuel [1 ]
机构：
[1] Inst Salud Carlos III, Serv Micol, Ctr Nacl Microbiol, Madrid, Spain
来源：
MEDICAL MYCOLOGY | 2012年 / 50卷 / 03期
关键词：
Early diagnosis; emerging pathogens; DNA detection; IN-VITRO; ALKALINE PROTEASE; ANTIFUNGAL AGENTS; FUNGAL PATHOGENS; RAPID DETECTION; ASPERGILLUS; IDENTIFICATION; DIAGNOSIS; MYCOLOGY; COMPLEX;
D O I：
10.3109/13693786.2011.604047
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
A duplex Real Time PCR (RT-PCR) assay for detecting DNA of members of the genus Fusarium has been developed and validated by using two mouse models of invasive infection. The duplex RT-PCR technique employed two specific molecular beacon probes targeting a highly conserved region of the fungal rDNA gene. This technique showed a detection limit of 10 fg DNA per mu l of sample and a specificity of 100%. The sensitivity in a total of 48 samples from a murine model of Fusarium solani infection was 93.9% for lung tissues and 86.7% for serum samples. In comparison, the sensitivity in a total of 45 samples of a F. oxysporum murine model infection was 87% for lung tissues and 42.8% for serum samples. This molecular technique could be a reliable method for the quantification and the evaluation of the disease in animal models and for the clinical diagnosis of fusariosis.
引用
收藏
页码：270 / 275
页数：6
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