ScDREB8, a novel A-5 type of DREB gene in the desert moss Syntrichia caninervis, confers salt tolerance to Arabidopsis

被引：41

作者：

Liang, Yuqing ^{[1
,2
]}

Li, Xiaoshuang ^{[1
]}

Zhang, Daoyuan ^{[1
]}

Gao, Bei ^{[3
,4
]}

Yang, Honglan ^{[1
]}

Wang, Yucheng ^{[1
]}

Guan, Kaiyun ^{[1
]}

Wood, Andrew J. ^{[5
]}

机构：

[1] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China

[4] Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Hong Kong, Peoples R China

[5] Southern Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2017年 / 120卷

关键词：

Syntrichia caninervis; Desiccation tolerance; DREB transcription factor; Salt stress; ROS-scavenging ability; BINDING TRANSCRIPTION FACTOR; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; STRESS TOLERANCE; LOW-TEMPERATURE; DROUGHT; EXPRESSION; OVEREXPRESSION; SYSTEM; DOMAIN;

D O I：

10.1016/j.plaphy.2017.09.014

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Salinity is a major limitation factor for crop productivity worldwide. DREB transcription factors broadly participate in plant stress response and have been extensively identified in a wide variety of plants. In this study, we characterized and analyzed the function of a novel A-5 type DREB gene ScDREB8 from the desiccation tolerant moss Syntrichia caninervis. Yeast one-hybrid experiment showed that ScDREB8 had no transactivation activity. Transient expression assay in onion epidermal cells revealed that ScDREB8 is distributed throughout the cell with no apparent specificity. Overexpression of ScDREB8 significantly increased the germination rate of Arabidopsis under salt stress and improved the salt tolerance of Arabidopsis at the seedling stage by up-regulating the expression of downstream stress-related genes and improving ROS scavenging ability. ScDREB8 is a promising candidate gene for improving crop salt stress and will provide greater insight to the molecular mechanism of stress tolerance of A-5 type DREB proteins. (C) 2017 Elsevier Masson SAS. All rights reserved.

引用

页码：242 / 251

页数：10

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