Development and characterization of two mini-X chromosomal short tandem repeat multiplexes

被引：19
作者：
Diegoli, Toni M. [1 ]
Coble, Michael D. [1 ]
机构：
[1] Armed Forces DNA Identificat Lab, Res Sect, Rockville, MD 20850 USA
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2011年 / 5卷 / 05期
关键词：
X chromosome; Mini-STRs; Identity testing; Multiplex PCR; Kinship testing; US population; AMELOGENIN SEX TEST; STR LOCI; GENETIC-ANALYSIS; FORENSIC CASEWORK; POPULATION-DATA; GENDER IDENTIFICATION; PRENATAL-DIAGNOSIS; GERMAN POPULATION; LINKED MARKERS; FREQUENCY DATA;
D O I：
10.1016/j.fsigen.2010.08.019
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Approximately 1360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200 pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples. Published by Elsevier Ireland Ltd.
引用
收藏
页码：415 / 421
页数：7
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