Fabrication, microstructure, and BMP-2 delivery of novel biodegradable and biocompatible silicate-collagen hybrid fibril sheets

被引：29

作者：

Chen, Song ^{[1
]}

Osaka, Akiyoshi ^{[2
]}

Ikoma, Toshiyuki ^{[3
]}

Morita, Hiromi ^{[1
]}

Li, Jie ^{[4
]}

Takeguchi, Masaki ^{[5
]}

Hanagata, Nobutaka ^{[1
]}

机构：

[1] Natl Inst Mat Sci, Ctr Biomat, Tsukuba, Ibaraki 3050047, Japan

[2] Okayama Univ, Grad Sch Nat Sci & Technol, Kita Ku, Okayama 700, Japan

[3] Tokyo Inst Technol, Dept Met & Ceram Sci, Meguro Ku, Tokyo 1528550, Japan

[4] Natl Inst Mat Sci, Nanotechnol Innovat Ctr, Tsukuba, Ibaraki 3050047, Japan

[5] Natl Inst Mat Sci, Adv Nanocharacterizat Ctr, Tsukuba, Ibaraki 3050003, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY | 2011年 / 21卷 / 29期

关键词：

GELATIN-SILOXANE HYBRIDS; BONE-FORMATION; GLUTARALDEHYDE; SCAFFOLDS; RHBMP-2; APATITE; CELLS; CYTOTOXICITY; OSTEOBLASTS; DEPOSITION;

D O I：

10.1039/c1jm10829h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Silicate-collagen hybrid fibril sheets were obtained using 3-glycidoxypropyl trimethoxysilane (GPTMS) as a novel cross-linker for type I collagen fibrils. BMP-2 was introduced in the hybrid sheets to stimulate cell proliferation and differentiation. Scanning electron microscopy images indicated that the resultant sheets were nanofibrous and porous. Transmission electron microscopy images showed that each hybrid fibril maintained a typical periodic unit of 60-70 nm. Compared to fibrils cross-linked by a conventional carbodiimide agent, the present hybrid fibrils were much more resistant against type I collagenase degradation. When soaked in BMP-2 solution, the hybrid sheets easily adsorbed BMP-2 and exhibited sustained release for BMP-2. Osteoblast-like MC3T3-E1 cells were well attached to and proliferated on the hybrid sheets. The proliferation and differentiation of these cells were enhanced to form collagen fibrils due to BMP-2 release.

引用

页码：10942 / 10948

页数：7

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