Microbial Communities Involved in Methane, Sulfur, and Nitrogen Cycling in the Sediments of the Barents Sea

被引:58
作者
Begmatov, Shahjahon [1 ]
Savvichev, Alexander S. [2 ]
Kadnikov, Vitaly V. [1 ]
Beletsky, Alexey, V [1 ]
Rusanov, Igor I. [2 ]
Klyuvitkin, Alexey A. [3 ]
Novichkova, Ekaterina A. [3 ]
Mardanov, Andrey, V [1 ]
Pimenov, Nikolai, V [2 ,4 ]
Ravin, Nikolai, V [1 ]
机构
[1] Russian Acad Sci, Res Ctr Biotechnol, Inst Bioengn, Moscow 119071, Russia
[2] Russian Acad Sci, Res Ctr Biotechnol, Winogradsky Inst Microbiol, Moscow 119071, Russia
[3] Russian Acad Sci, Shirshov Inst Oceanol, Moscow 117997, Russia
[4] Russian Acad Sci, Ilichev Pacific Inst Oceanol, Far East Branch, Vladivostok 690041, Russia
基金
俄罗斯科学基金会; 俄罗斯基础研究基金会;
关键词
arctic; marine sediments; methane cycle; sulfur cycle; nitrogen cycle; microbial communities; Barents Sea; SULFATE-REDUCING BACTERIA; MOSBY MUD VOLCANO; ANAEROBIC OXIDATION; ARCTIC-OCEAN; GEN; NOV; ELEMENTAL SULFUR; FLOOR SEDIMENTS; ORGANIC-CARBON; MATTER; WATER;
D O I
10.3390/microorganisms9112362
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A combination of physicochemical and radiotracer analysis, high-throughput sequencing of the 16S rRNA, and particulate methane monooxygenase subunit A (pmoA) genes was used to link a microbial community profile with methane, sulfur, and nitrogen cycling processes. The objects of study were surface sediments sampled at five stations in the northern part of the Barents Sea. The methane content in the upper layers (0-5 cm) ranged from 0.2 to 2.4 mu M and increased with depth (16-19 cm) to 9.5 mu M. The rate of methane oxidation in the oxic upper layers varied from 2 to 23 nmol CH4 L-1 day(-1) and decreased to 0.3 nmol L-1 day(-1) in the anoxic zone at a depth of 16-19 cm. Sulfate reduction rates were much higher, from 0.3 to 2.8 mu mol L-1 day(-1). In the surface sediments, ammonia-oxidizing Nitrosopumilaceae were abundant; the subsequent oxidation of nitrite to nitrate can be carried out by Nitrospira sp. Aerobic methane oxidation could be performed by uncultured deep-sea cluster 3 of gamma-proteobacterial methanotrophs. Undetectable low levels of methanogenesis were consistent with a near complete absence of methanogens. Anaerobic methane oxidation in the deeper sediments was likely performed by ANME-2a-2b and ANME-2c archaea in consortium with sulfate-reducing Desulfobacterota. Sulfide can be oxidized by nitrate-reducing Sulfurovum sp. Thus, the sulfur cycle was linked with the anaerobic oxidation of methane and the nitrogen cycle, which included the oxidation of ammonium to nitrate in the oxic zone and denitrification coupled to the oxidation of sulfide in the deeper sediments. Methane concentrations and rates of microbial biogeochemical processes in sediments in the northern part of the Barents Sea were noticeably higher than in oligotrophic areas of the Arctic Ocean, indicating that an increase in methane concentration significantly activates microbial processes.
引用
收藏
页数:21
相关论文
共 112 条
[31]   Effects of climate change on methane emissions from seafloor sediments in the Arctic Ocean: A review [J].
James, Rachael H. ;
Bousquet, Philippe ;
Bussmann, Ingeborg ;
Haeckel, Matthias ;
Kipfer, Rolf ;
Leifer, Ira ;
Niemann, Helge ;
Ostrovsky, Ilia ;
Piskozub, Jacek ;
Rehder, Gregor ;
Treude, Tina ;
Vielstaedte, Lisa ;
Greinert, Jens .
LIMNOLOGY AND OCEANOGRAPHY, 2016, 61 :S283-S299
[32]   Development of a novel methanotrophic process with the helper micro-organism Hyphomicrobium sp. NM3 [J].
Jeong, S. -Y. ;
Kim, T. G. .
JOURNAL OF APPLIED MICROBIOLOGY, 2019, 126 (02) :534-544
[33]   METHANE OXIDATION BY NITROSOCOCCUS-OCEANUS AND NITROSOMONAS-EUROPAEA [J].
JONES, RD ;
MORITA, RY .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (02) :401-410
[34]   The Biogeochemical Sulfur Cycle of Marine Sediments [J].
Jorgensen, Bo Barker ;
Findlay, Alyssa J. ;
Pellerin, Andre .
FRONTIERS IN MICROBIOLOGY, 2019, 10
[35]   Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle [J].
Kadnikov, Vitaly V. ;
Mardanov, Andrey V. ;
Beletsky, Alexey V. ;
Karnachuk, Olga V. ;
Ravin, Nikolai V. .
EXTREMOPHILES, 2019, 23 (02) :189-200
[36]   The structure of bacterial communities in the western Arctic Ocean as revealed by pyrosequencing of 16S rRNA genes [J].
Kirchman, David L. ;
Cottrell, Matthew T. ;
Lovejoy, Connie .
ENVIRONMENTAL MICROBIOLOGY, 2010, 12 (05) :1132-1143
[37]   On the evolution and physiology of cable bacteria [J].
Kjeldsen, Kasper U. ;
Schreiber, Lars ;
Thorup, Casper A. ;
Boesen, Thomas ;
Bjerg, Jesper T. ;
Yang, Tingting ;
Dueholm, Morten S. ;
Larsen, Steffen ;
Risgaard-Petersen, Nils ;
Nierychlo, Marta ;
Schmid, Markus ;
Boggild, Andreas ;
van de Vossenberg, Jack ;
Geelhoed, Jeanine S. ;
Meysman, Filip J. R. ;
Wagner, Michael ;
Nielsen, Per H. ;
Nielsen, Lars Peter ;
Schramm, Andreas .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (38) :19116-19125
[38]   Distribution and in situ abundance of sulfate-reducing bacteria in diverse marine hydrocarbon seep sediments [J].
Kleindienst, Sara ;
Ramette, Alban ;
Amann, Rudolf ;
Knittel, Katrin .
ENVIRONMENTAL MICROBIOLOGY, 2012, 14 (10) :2689-2710
[39]   Studies of the European Arctic on Cruise 80 of the R/V Akademik Mstislav Keldysh [J].
Klyuvitkin, A. A. ;
Politova, N., V ;
Novigatsky, A. N. ;
Kravchishina, M. D. .
OCEANOLOGY, 2021, 61 (01) :139-141
[40]   Diversity and Habitat Preferences of Cultivated and Uncultivated Aerobic Methanotrophic Bacteria Evaluated Based on pmoA as Molecular Marker [J].
Knief, Claudia .
FRONTIERS IN MICROBIOLOGY, 2015, 6