Effects of nitrogen source availability and bioreactor operating strategies on lutein production with Scenedesmus obliquus FSP-3

被引：51

作者：

Ho, Shih-Hsin ^{[1
]}

Xie, Youping ^{[2
]}

Chan, Ming-Chang ^{[3
]}

Liu, Chen-Chun ^{[4
]}

Chen, Chun-Yen ^{[4
]}

Lee, Duu-Jong ^{[5
]}

Huang, Chieh-Chen ^{[6
]}

Chang, Jo-Shu ^{[3
,4
,7
,8
]}

机构：

[1] Kobe Univ, Org Adv Sci & Technol, Kobe, Hyogo 6578501, Japan

[2] Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China

[3] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan

[4] Natl Cheng Kung Univ, Ctr Biosci & Biotechnol, Tainan 701, Taiwan

[5] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei, Taiwan

[6] Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung 40227, Taiwan

[7] Natl Cheng Kung Univ, Res Ctr Energy Technol, Tainan 701, Taiwan

[8] Natl Cheng Kung Univ, Strategy Ctr, Tainan 701, Taiwan

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 184卷

关键词：

Microalgae; Scenedesmus obliquus; Lutein; Light intensity shift; Bioreactor strategy; CHLORELLA-PROTOTHECOIDES; LIPID-ACCUMULATION; LIGHT-INTENSITY; CELL-GROWTH; WASTE-WATER; CNW-N; CAROTENOIDS; MICROALGAE; BIOMASS; CULTURE;

D O I：

10.1016/j.biortech.2014.10.062

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, the effects of the type and concentration of nitrogen sources on the cell growth and lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest lutein content (4.61 mg/g) and lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and lutein productivity (6.01 mg/L/day). This performance is better than most related studies. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：131 / 138

页数：8

共 35 条

[1] Food carotenoids and cancer prevention: An overview of current research [J].

Astorg, P .

TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1997, 8 (12) :406-413

[2]

BOROWITZKA MA, 1991, J APPL PHYCOL, V3, P295, DOI 10.1007/BF00026091

[3]

Butler WR, 1996, J ANIM SCI, V74, P858

[4] Recovery of Lutein from Microalgae Biomass: Development of a Process for Scenedesmus almeriensis Biomass [J].

Ceron, M. Carmen ;

Campos, Inmaculada ;

Sanchez, Juan F. ;

Acien, Francisco G. ;

Molina, Emilio ;

Fernandez-Sevilla, Jose M. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (24) :11761-11766

[5] Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N [J].

Chan, Ming-Chang ;

Ho, Shih-Hsin ;

Lee, Duu-Jong ;

Chen, Chun-Yen ;

Huang, Chieh-Chen ;

Chang, Jo-Shu .

BIOCHEMICAL ENGINEERING JOURNAL, 2013, 78 :24-31

[6] Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation [J].

Cheirsilp, Benjamas ;

Torpee, Salwa .

BIORESOURCE TECHNOLOGY, 2012, 110 :510-516

[7] Nutritional antioxidants and age-related cataract and maculopathy [J].

Chiu, Chung-Jung ;

Taylor, Allen .

EXPERIMENTAL EYE RESEARCH, 2007, 84 (02) :229-245

[8] The air-lift photobioreactors with flow patterning for high-density cultures of microalgae and carbon dioxide removal [J].

Chiu, Sheng-Yi ;

Tsai, Ming-Ta ;

Kao, Chien-Ya ;

Ong, Seow-Chin ;

Lin, Chih-Sheng .

ENGINEERING IN LIFE SCIENCES, 2009, 9 (03) :254-260

[9] Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis [J].

Cordero, Baldo F. ;

Obraztsova, Irina ;

Couso, Inmaculada ;

Leon, Rosa ;

Angeles Vargas, Maria ;

Rodriguez, Herminia .

MARINE DRUGS, 2011, 9 (09) :1607-1624

[10] Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta) [J].

Del Campo, JA ;

Rodríguez, H ;

Moreno, J ;

Vargas, MA ;

Rivas, J ;

Guerrero, MG .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (06) :848-854

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