Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases

被引：28
作者：
Serim, Sevnur [1 ]
Baer, Philipp [1 ]
Verhelst, Steven H. L. [1 ,2 ]
机构：
[1] Tech Univ Munich, Lehrstuhl Chem Biopolymere, D-85354 Freising Weihenstephan, Germany
[2] ISAS eV, Leibniz Inst Analyt Sci, D-44227 Dortmund, Germany
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2015年 / 13卷 / 08期
关键词：
DERIVATIVES; ISOCOUMARINS; INHIBITION; PROTEOMICS; ROUTE;
D O I：
10.1039/c4ob02444c
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for serine proteases based on a phosphonate ester scaffold.
引用
收藏
页码：2293 / 2299
页数：7
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