Effect of linear alkylbenzene sulfonate (LAS) on human intestinal Caco-2 cells at non cytotoxic concentrations

被引:13
作者
Bradai, Mohamed [1 ]
Han, Junkyu [2 ,3 ]
El Omri, Abdelfatteh [2 ]
Funamizu, Naoyuki [4 ]
Sayadi, Sami [5 ]
Isoda, Hiroko [2 ,3 ]
机构
[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tennodai 1-1-1, Tsukuba, Ibaraki 3058572, Japan
[2] Univ Tsukuba, Fac Life & Environm Sci, Tennodai 1-1-1, Tsukuba, Ibaraki 3058572, Japan
[3] Univ Tsukuba, Alliance Res North Africa, Tennodai 1-1-1, Tsukuba, Ibaraki 3058572, Japan
[4] Hokkaido Univ, Grad Sch Engn, Sapporo, Hokkaido 0600808, Japan
[5] Biotechnol Ctr Sfax, Lab Environm Bioproc, Sfax 3018, PB, Tunisia
基金
日本学术振兴会; 日本科学技术振兴机构;
关键词
Linear alkylbenzene sulfonate; Caco-2; cells; Cell proliferation; Proteomics; Elongation factor-2; Dipeptidyl peptidase 3; 14-3-3 Protein theta; DIPEPTIDYL PEPTIDASE-III; TRANSCRIPTION FACTOR; KAPPA-B; PROLIFERATION; EXPRESSION; GROWTH; 14-3-3-PROTEINS; 14-3-3-SIGMA; PROGRESSION; APOPTOSIS;
D O I
10.1007/s10616-015-9887-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Linear alkylbenzene sulfonate (LAS) is a cytotoxic synthetic anionic surfactant widely present in the environment due to its large-scale production and intensive use in the detergency field. In this study, we investigated the effect of LAS (CAS No. 25155-30-0) at non cytotoxic concentrations on human intestinal Caco-2 cells using different in vitro bioassays. As results, LAS increased Caco-2 cell proliferation at concentrations ranging from 1 to 15 ppm, more significantly for shorter exposure time (24 h), confirmed using flow cytometry and trypan blue exclusion methods. Moreover, proteomics analysis revealed that this effect was associated with an over-expression of elongation factor 2 and dipeptidyl peptidase 3, and a down-regulation of 14-3-3 protein theta, confirmed at mRNA level using real-time PCR. These findings suggest that LAS at non cytotoxic concentrations, similar to those observed at wastewater treatment plants outlets, increases the growth rate of colon cancer cells, raising thereby its tumor promotion effect potential.
引用
收藏
页码:1267 / 1275
页数:9
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