A vector for the expression of recombinant monoclonal Fab fragments in bacteria

被引：22

作者：

Burioni, R

Plaisant, P

Bugli, F

Delli Carri, V

Clementi, M

Fadda, G

机构：

[1] Univ Cattolica Sacro Cuore, Ist Microbiol, I-00168 Rome, Italy

[2] Univ Trieste, Dipartimento Med Sperimentale, Trieste, Italy

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1998年 / 217卷 / 1-2期

关键词：

monoclonal recombinant antibodies; combinatorial libraries;

D O I：

10.1016/S0022-1759(98)00102-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The availability of genes coding for monoclonal Fab fragments of a desired specificity permits their expression in bacteria and provides a simple method for the generation of good quality reagents. in this paper we describe a new phagemid vector for the production of recombinant Fabs from penes obtained from phage display combinatorial libraries. The phagemid features an antibiotic resistance cassette which: once inserted between the heavy chain fragment and the light chain genes, avoids unwanted recombination and preserves useful restriction sites not affecting the Fab production rate. (C) 1998 Elsevier Science B.V. All rights reserved.

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页码：195 / 199

页数：5

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