A monoclonal antibody that enables specific immunohistological detection of prion protein in bovine spongiform encephalopathy cases

被引：6
|
作者：
Laffling, AJ [1 ]
Baird, A [1 ]
Birkett, CR [1 ]
John, HA [1 ]
机构：
[1] Moredun Res Inst, Int Res Ctr, Penicuik EH26 0PZ, Midlothian, Scotland
来源：
NEUROSCIENCE LETTERS | 2001年 / 300卷 / 02期
关键词：
bovine spongiform encephalopathy; scrapie; medulla oblongata; immunohistology; prion protein; monoclonal antibody KG9;
D O I：
10.1016/S0304-3940(01)01562-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The specificity of a monoclonal antibody (mAB) raised against recombinant bovine prion protein (PrP) for the immunohistological detection of PrP accumulation in the medulla oblongata of bovine spongiform encephalopathy (BSE) and ovine scrapie cases was investigated. mAB KG9 showed a diffuse low intensity reaction with the cytoplasm of neurones in normal cattle and sheep sections. In BSE sections the mAB detected widespread granular deposits of PrP associated with neurones and the neuropil. Although scrapie sections showed similar levels of granular deposits with another antibody to PrP these were not detected by KG9 which did however detect diffuse staining in neuronal cytoplasm. Possible explanations for the specificity of binding of KG9 are discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：99 / 102
页数：4
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