A monoclonal antibody that enables specific immunohistological detection of prion protein in bovine spongiform encephalopathy cases

被引：6
|
作者：
Laffling, AJ [1 ]
Baird, A [1 ]
Birkett, CR [1 ]
John, HA [1 ]
机构：
[1] Moredun Res Inst, Int Res Ctr, Penicuik EH26 0PZ, Midlothian, Scotland
来源：
NEUROSCIENCE LETTERS | 2001年 / 300卷 / 02期
关键词：
bovine spongiform encephalopathy; scrapie; medulla oblongata; immunohistology; prion protein; monoclonal antibody KG9;
D O I：
10.1016/S0304-3940(01)01562-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The specificity of a monoclonal antibody (mAB) raised against recombinant bovine prion protein (PrP) for the immunohistological detection of PrP accumulation in the medulla oblongata of bovine spongiform encephalopathy (BSE) and ovine scrapie cases was investigated. mAB KG9 showed a diffuse low intensity reaction with the cytoplasm of neurones in normal cattle and sheep sections. In BSE sections the mAB detected widespread granular deposits of PrP associated with neurones and the neuropil. Although scrapie sections showed similar levels of granular deposits with another antibody to PrP these were not detected by KG9 which did however detect diffuse staining in neuronal cytoplasm. Possible explanations for the specificity of binding of KG9 are discussed. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：99 / 102
页数：4
相关论文
共 50 条
  • [31] Bovine Spongiform Encephalopathy Induces Misfolding of Alleged Prion-Resistant Species Cellular Prion Protein without Altering Its Pathobiological Features
    Vidal, Enric
    Fernandez-Borges, Natalia
    Pintado, Belen
    Ordonez, Montserrat
    Marquez, Mercedes
    Fondevila, Dolors
    Maria Torres, Juan
    Pumarola, Marti
    Castilla, Joaquin
    JOURNAL OF NEUROSCIENCE, 2013, 33 (18): : 7778 - 7786
  • [32] Antigen Retrieval Using Sodium Hydroxide for Prion Immunohistochemistry in Bovine Spongiform Encephalopathy and Scrapie
    Okada, H.
    Sato, Y.
    Sata, T.
    Sakurai, M.
    Endo, J.
    Yokoyama, T.
    Mohri, S.
    JOURNAL OF COMPARATIVE PATHOLOGY, 2011, 144 (04) : 251 - 256
  • [33] Evidence of subclinical prion disease in aged mice following exposure to bovine spongiform encephalopathy
    Brown, Karen L.
    Mabbott, Neil A.
    JOURNAL OF GENERAL VIROLOGY, 2014, 95 : 231 - 243
  • [34] Influence of Interspecies Transmission of Atypical Bovine Spongiform Encephalopathy Prions to Hamsters on Prion Characteristics
    Miyazawa, Kohtaro
    Masujin, Kentaro
    Matsuura, Yuichi
    Iwamaru, Yoshifumi
    Okada, Hiroyuki
    FRONTIERS IN VETERINARY SCIENCE, 2020, 7
  • [35] Bovine spongiform encephalopathy in Japan: History and recent studies on oxidative stress in prion diseases
    Onodera, Takashi
    Sakudo, Akikazu
    Wu, Guoying
    Saeki, Keiichi
    MICROBIOLOGY AND IMMUNOLOGY, 2006, 50 (08) : 565 - 578
  • [36] Classical bovine spongiform encephalopathy and chronic wasting disease: two sides of the prion coin
    Haley, Nicholas J.
    Richt, Juergen A.
    ANIMAL DISEASES, 2023, 3 (01):
  • [37] Prion protein gene sequence of Canada's first non-imported case of bovine spongiform encephalopathy (BSE)
    Coulthart, MB
    Mogk, R
    Rancourt, JM
    Glodal, DL
    Czub, S
    GENOME, 2003, 46 (06) : 1005 - 1009
  • [38] Neuroanatomical Distribution of Disease-Associated Prion Protein in Experimental Bovine Spongiform Encephalopathy in Cattle after Intracerebral Inoculation
    Fukuda, Shigeo
    Onoe, Sadao
    Nikaido, Satoshi
    Fujii, Kei
    Kageyama, Soichi
    Iwamaru, Yoshifumi
    Imamura, Morikazu
    Masujin, Kentaro
    Matsuura, Yuichi
    Shimizu, Yoshihisa
    Kasai, Kazuo
    Yoshioka, Miyako
    Murayama, Yuichi
    Mohri, Shirou
    Yokoyama, Takashi
    Okada, Hiroyuki
    JAPANESE JOURNAL OF INFECTIOUS DISEASES, 2012, 65 (01) : 37 - 44
  • [39] Abnormal prion protein is associated with changes of plasma membranes and endocytosis in bovine spongiform encephalopathy (BSE)-affected cattle brains
    Ersdal, C.
    Goodsir, C. M.
    Simmons, M. M.
    McGovern, G.
    Jeffrey, M.
    NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 2009, 35 (03) : 259 - 271
  • [40] Two Unusual Bovine Spongiform Encephalopathy Cases Detected in Great Britain
    Stack, M.
    Focosi-Snyman, R.
    Cawthraw, S.
    Davis, L.
    Jenkins, R.
    Thorne, L.
    Chaplin, M.
    Everitt, S.
    Saunders, G.
    Terry, L.
    ZOONOSES AND PUBLIC HEALTH, 2009, 56 (6-7) : 376 - 383
12345