A target specific pathway from nitric oxide synthase immunoreactive preganglionic sympathetic to superior cervical ganglion neurons innervating the submandibular salivary gland

The correlations between nitric oxide synthase (NOS)-immunoreactive (NOS-ir) preganglionic sympathetic neurons (PSNs) in the thoracic spinal cord and the neurons in the superior cervical ganglia (SCG) were studied with special reference to target specificity. NOS-ir neurons were distributed in the intermediate gray of the spinal cord and most numerous in the intermediolateral subnucleus of the PSNs. NOS-ir PSNs received direct synaptic contacts from tyrosine hydroxylase-ir, 5-hydroxytryptamine-ir, gamma-amino butyric acid-ir, and phosphate-activated glutaminase-ir axons. A majority of PSNs projecting to SCG were NOS-ir but some were NOS-negative. Large SCG neurons surrounded by a dense network of NOS-ir axons from PSNs projected to the submandibular salivary gland. NPY-ir small SCG neurons devoid of NOS-ir PSN innervation projected to blood vessels. SIF cells in the SCG were NOS-negative and provided with a meshwork of NOS-ir axons. The present results suggest a subpopulation of SCG neurons may be concerned with a distinct functional category in the rat under the influence of NOS-ir preganglionic sympathetic neurons. (C) 1998 Elsevier Science B.V. All rights reserved.