Characterization of enterohemorrhagic Escherichia coli O157:H7 00B015: a Shiga toxin producing but virulence-attenuated isolate

被引：0
作者：
Wang, Haiguang [1 ]
Gu, Jiang [1 ]
Yu, Shu [1 ]
Zhang, Weijun [1 ]
Zhu, Yefei [2 ]
Zou, Quanming [1 ]
Zhu, Fengcai [2 ]
Mao, Xuhu [1 ]
机构：
[1] Third Mil Med Univ, Dept Clin Microbiol & Immunol, Coll Med Lab Sci, Chongqing 400038, Peoples R China
[2] Jiangsu Prov Ctr Dis Control & Prevent, Nanjing 210009, Peoples R China
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 2010年 / 56卷 / 08期
基金：
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词：
enterohemorrhagic Escherichia coli; Shiga toxin; actin polymerization; ACTIN POLYMERIZATION; HEMORRHAGIC COLITIS; O157-H7; TIR; INTIMIN; INFECTIONS; PROTECTION; EFFECTOR; ESPF(U); VACCINE;
D O I：
10.1139/W10-049
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Enterohemorrhagic Escherichia coli (EHEC) causes a wide range of systematic diseases in human and animals in 2 main ways: (1) production of Shiga toxin (Stx) and (2) induction of actin polymerization characterized by attaching and effacing (A/E) lesions. Stx is commonly targeted in the development of drugs and vaccines to control EHEC infection for its indispensible contribution to EHEC pathogenisis. In this study, we isolated a Stx-producing EHEC O157:H7 isolate 00B015 and found that its ability to induce actin polymerization was impaired. In addition, it reduces pathogenicity and decreases mortality in mice. Our results report a Stx-producing but virulence-attenuated EHEC isolate 00B015 and suggest that the formation of actin polymerization may help Stx-induced pathogenesis and have a more important contribution in EHEC infections.
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页码：651 / 656
页数：6
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